Maloof Lab:Protocols

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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Protocols

DNA extraction for 96 well plates, CTAB method

DNA precipitation for 96 well plates, with glycogen

E. coli (DH5α chemically-competent) transformation

Agrobacterium transformation (Freeze-Thaw method)

Cleaning ball bearings

Seed sterilization

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Exo-SAP PCR cleanup for sequencing

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

Trizol extraction

Stocks

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl₂

Lab Safety

Maloof lab safety

Lab Tasks

Maloof lab tasks

Scripts, instructions and miscellaneous

Using the LI1800 light meter

Sequence Analysis with eBioX

Analyzing luminiscence over time with ImageJ

Hypocotyl measurement with ImageJ

Maloof/Harmer Primer Database Search (Only internal users)

Patricia's Motif search (Only internal users)

Laser Tough Tag Settings to print

PDA measurement system

Measuring time-lapse hypocotyl movies with ImageJ

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