Maloof Lab:Seed Storage: Difference between revisions

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(New page: *Let seed dry on plant until siliques are completely brown (but before they split and drop all of their seed) *Collect seed and place an alliquot in a 1.5ml screwcap eppondorf tube *Create...)
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Revision as of 14:30, 1 August 2008

  • Let seed dry on plant until siliques are completely brown (but before they split and drop all of their seed)
  • Collect seed and place an alliquot in a 1.5ml screwcap eppondorf tube
  • Create a database entry for your seed in the seed database
  • Place eppendorf tube in dessicator
  • Keep in dessicator for two weeks 'after' dessicator reaches 15% humidity
  • Remove from dessicator and cap quickly
  • Store at -20°C
  • Let tube warm to room temperature before opening