Maloof Lab:Trizol: Difference between revisions
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#Incubate 10 minutes at room temperature | #Incubate 10 minutes at room temperature | ||
#Centrifuge 12000 rpm for 10 minutes at 4°C | #Centrifuge 12000 rpm for 10 minutes at 4°C | ||
#Discard supernatant and wash pellet in 1 ml 75% ethanol | #Discard supernatant and wash pellet in 1 ml 75% ethanol (made with DEPC water) | ||
#Discard supernatant and wash pellet in 1 ml 75% ethanol | #Discard supernatant and wash pellet in 1 ml 75% ethanol (made with DEPC water) | ||
#Centrifuge at 7000 rpm for 5 minutes at 4°C | #Centrifuge at 7000 rpm for 5 minutes at 4°C | ||
#Air dry for 10 minutes | #Air dry for 10 minutes | ||
#Resuspend in 30μl | #Resuspend in 30μl DEPC water | ||
#Incubate 10 minutes at 55°C | #Incubate 10 minutes at 55°C | ||
#Store at -80°C | #Store at -80°C | ||
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#Add 1ml DEPC to 1L milli-Q water<br> | #Add 1ml DEPC to 1L milli-Q water<br> | ||
#Let sit overnight | #Let sit overnight | ||
#Autoclave tha next day | #Autoclave tha next day | ||
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Revision as of 17:23, 17 June 2009
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Room 2115 |
RNA extraction with TRIzol (invitrogen cat# 15596-026)
1L DEPC water
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