Maloof Lab:Trizol: Difference between revisions
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#Incubate 10 minutes at room temperature | #Incubate 10 minutes at room temperature | ||
#Centrifuge 12000 rpm for 10 minutes at 4°C | #Centrifuge 12000 rpm for 10 minutes at 4°C | ||
#Discard supernatant and wash pellet in 1 ml 75% ethanol (made with DEPC water) | #Discard supernatant and wash pellet in 1 ml 75% ethanol (made with DEPC water) | ||
#Centrifuge at 7000 rpm for 5 minutes at 4°C | #Centrifuge at 7000 rpm for 5 minutes at 4°C | ||
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#Incubate 10 minutes at 55°C | #Incubate 10 minutes at 55°C | ||
#Store at -80°C | #Store at -80°C | ||
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<h5>extra steps to have a cleaner sample</h5> | |||
#After step 13 add 3μl NaOAc | |||
#Add 60μl ethanol 100% | |||
#Incubate at -80°C for 30 minutes | |||
#centrifuge at maximum speed for 30 minutes at 4°C | |||
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<h3><font style="color:#F8B603;">1L DEPC water</font></h3> | <h3><font style="color:#F8B603;">1L DEPC water</font></h3> |
Latest revision as of 17:35, 17 June 2009
Room 2115 |
RNA extraction with TRIzol (invitrogen cat# 15596-026)
extra steps to have a cleaner sample
1L DEPC water
|