Maloof Lab:antibiotics: Difference between revisions
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(New page: {{Template:Maloof_Lab}} {| class="wikitable" style="text-align:left" |+<h3><font style="color:#F8B603;">Antibiotic stocks</font></h3> |- !antibiotic!!bgcolor="red"|abb!!stock conc.!!LB pla...) |
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|+<h3><font style="color:#F8B603;">Antibiotic stocks</font></h3> | |+<h3><font style="color:#F8B603;">Antibiotic stocks</font></h3> | ||
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! | ! !!bgcolor="#C9D3EB"|abb!!bgcolor="#C9D3EB"|stock conc.!!bgcolor="#C9D3EB"|LB plate<br> conc.!!bgcolor="#C9D3EB"|Vol. in 500ml<br>LB agar!!bgcolor="#C9D3EB"|MS plate<br> conc!!bgcolor="#C9D3EB"|Vol. in<br>1L MS | ||
|- | |- | ||
!ampicilin | !bgcolor="#C9D3EB"|ampicilin | ||
|ap||50 mg/ml||100 | |ap||50 mg/ml||100 μg/ml||1 ml|| || | ||
|- | |- | ||
!kanamycin | !bgcolor="#C9D3EB"|kanamycin | ||
|km/kn||50 mg/ml||50 | |km/kn||50 mg/ml||50 μg/ml||0.5 ml||50 μg/ml||1 ml | ||
|- | |- | ||
! | !bgcolor="#C9D3EB"|spectinomycin | ||
|sp|| | |sp||100 mg/ml||100 μg/ml||0.5 ml|| || | ||
|- | |- | ||
!chloramphenicol | !bgcolor="#C9D3EB"|chloramphenicol | ||
|cm||34 mg/ml in ethanol||34 | |cm||34 mg/ml in ethanol||34 μg/ml||0.5 ml|| || | ||
|- | |- | ||
!rifampicin | !bgcolor="#C9D3EB"|rifampicin | ||
|rif||34 mg/ml in methanol||150 | |rif||34 mg/ml in methanol||150 μg/ml||2.2 ml|| || | ||
|- | |- | ||
!tetracycline | !bgcolor="#C9D3EB"|tetracycline | ||
|tc||12 mg/ml||12 | |tc/tet||12 mg/ml||12 μg/ml||0.5 ml|| || | ||
|- | |- | ||
!hygromycin | !bgcolor="#C9D3EB"|hygromycin | ||
|hyg||20 mg/ml|| || ||20 | |hyg||20 mg/ml|| || ||20 μg/ml||1 ml | ||
|- | |- | ||
!carbenicillin | !bgcolor="#C9D3EB"|carbenicillin | ||
|carb||100 mg/ml|| || ||500 | |carb||100 mg/ml|| || ||500 μg/ml||5 ml | ||
|- | |- | ||
!thiamine | !bgcolor="#C9D3EB"|thiamine | ||
| ||50 mg/ml|| || ||50 | | ||50 mg/ml|| || ||50 μg/ml||1 ml | ||
|- | |- | ||
!gentamycin* | !bgcolor="#C9D3EB"|gentamycin* | ||
|gent||effective dose 6.5 mg/ml||15 | |gent||effective dose 6.5 mg/ml||15 μg/ml||0.75 ml||75 μg/ml||1 ml | ||
|- | |- | ||
!basta | !bgcolor="#C9D3EB"|basta | ||
|| || | || ||20 mg/ml|| || ||20 μg/ml||1 ml | ||
|- | |- | ||
!zeocin | !bgcolor="#C9D3EB"|zeocin | ||
||Zeo||100 mg/ml||25 | ||Zeo||100 mg/ml||25 μg/ml||0.125 ml|| || | ||
|- | |- | ||
|colspan="7"|* the potency of gentamycin varies from stock to stock, the volume added must be calculated according to the potency of the stock | |colspan="7"|* the potency of gentamycin varies from stock to stock, the volume added must be calculated according to the potency of the stock | ||
|} | |} |
Latest revision as of 13:30, 6 December 2011
Room 2115 |
abb | stock conc. | LB plate conc. |
Vol. in 500ml LB agar |
MS plate conc |
Vol. in 1L MS | |
---|---|---|---|---|---|---|
ampicilin | ap | 50 mg/ml | 100 μg/ml | 1 ml | ||
kanamycin | km/kn | 50 mg/ml | 50 μg/ml | 0.5 ml | 50 μg/ml | 1 ml |
spectinomycin | sp | 100 mg/ml | 100 μg/ml | 0.5 ml | ||
chloramphenicol | cm | 34 mg/ml in ethanol | 34 μg/ml | 0.5 ml | ||
rifampicin | rif | 34 mg/ml in methanol | 150 μg/ml | 2.2 ml | ||
tetracycline | tc/tet | 12 mg/ml | 12 μg/ml | 0.5 ml | ||
hygromycin | hyg | 20 mg/ml | 20 μg/ml | 1 ml | ||
carbenicillin | carb | 100 mg/ml | 500 μg/ml | 5 ml | ||
thiamine | 50 mg/ml | 50 μg/ml | 1 ml | |||
gentamycin* | gent | effective dose 6.5 mg/ml | 15 μg/ml | 0.75 ml | 75 μg/ml | 1 ml |
basta | 20 mg/ml | 20 μg/ml | 1 ml | |||
zeocin | Zeo | 100 mg/ml | 25 μg/ml | 0.125 ml | ||
* the potency of gentamycin varies from stock to stock, the volume added must be calculated according to the potency of the stock |