Maloof Lab:gelred loading dye: Difference between revisions

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To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer.
To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer.
This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.


#5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)
#5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)
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To be written
Use the Fermentas GeneRuler 1kb ladder from the stock. Mix:


#100µl (1 tube) DNA Ladder
#100µl 6X Loading Dye Solution with GelRed
#400µl Deionized water
Good to go. I typically load 3µl.
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Revision as of 12:35, 18 May 2009

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Loading Buffer with GelRed for Electrophoresis

To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.

  1. 5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)
  2. 15 ul of GelRed (10000x in water, cat.41003 Biotium)


1 Kb Ladder with GelRed for Electrophoresis

Use the Fermentas GeneRuler 1kb ladder from the stock. Mix:

  1. 100µl (1 tube) DNA Ladder
  2. 100µl 6X Loading Dye Solution with GelRed
  3. 400µl Deionized water

Good to go. I typically load 3µl.

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