Maloof Lab:gelred loading dye: Difference between revisions
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<h3><font style="color:#F8B603;">6x Xylene Cyanol Loading Buffer for Electrophoresis</font></h3> | |||
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This buffer will have only the <b>light</b> blue than runs <b>slower</b> on the gel. | |||
This protocol will make 40ml of 6x solution. Adjust to your needs if necessary. | |||
#100mg Xylene-cyanol | |||
#6g of Ficoll (Type 400) | |||
#Add water to 40 ml | |||
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. | |||
<h3><font style="color:#C9D3EB;">6x Bromophenol Blue Loading Buffer for Electrophoresis</font></h3> | |||
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This protocol will make 2 liter of 50x solution. Adjust to your needs if necessary. | |||
This buffer will have only the <b>dark</b> blue than runs <b>faster</b> on the gel. | |||
This protocol will make 40ml of 6x solution. Adjust to your needs if necessary. | |||
#100mg Bromophenol Blue | |||
#6g of Ficoll (Type 400) | |||
#Add water to 40 ml | |||
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. | |||
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<h3><font style="color:#C9D3EB;">Loading Buffer with GelRed for Electrophoresis</font></h3> | <h3><font style="color:#C9D3EB;">Loading Buffer with GelRed for Electrophoresis</font></h3> | ||
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To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer. | To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. | ||
This protocol will make 5ml of 6x solution. Adjust to your needs if necessary. | This protocol will make 5ml of 6x solution. Adjust to your needs if necessary. | ||
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#100µl (1 tube) DNA Ladder | #100µl (1 tube) DNA Ladder | ||
#100µl 6X Loading Dye Solution with GelRed | #100µl 6X Loading Dye Solution with GelRed (from above) | ||
#400µl Deionized water | #400µl Deionized water | ||
Good to go. | Good to go. Load 3 to 5 µl. | ||
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Revision as of 12:38, 18 May 2009
Room 2115 |
6x Xylene Cyanol Loading Buffer for ElectrophoresisThis buffer will have only the light blue than runs slower on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. 6x Bromophenol Blue Loading Buffer for ElectrophoresisThis protocol will make 2 liter of 50x solution. Adjust to your needs if necessary. This buffer will have only the dark blue than runs faster on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. |
Loading Buffer with GelRed for Electrophoresis
To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.
- 5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)
- 15 ul of GelRed (10000x in water, cat.41003 Biotium)
1 Kb Ladder with GelRed for Electrophoresis
Use the Fermentas GeneRuler 1kb ladder from the stock. Mix:
- 100µl (1 tube) DNA Ladder
- 100µl 6X Loading Dye Solution with GelRed (from above)
- 400µl Deionized water
Good to go. Load 3 to 5 µl.
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