Maloof Lab:gelred loading dye: Difference between revisions
No edit summary |
No edit summary |
||
Line 29: | Line 29: | ||
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. | Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. | ||
Revision as of 12:39, 18 May 2009
Room 2115 |
6x Xylene Cyanol Loading Buffer for ElectrophoresisThis buffer will have only the light blue than runs slower on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. 6x Bromophenol Blue Loading Buffer for ElectrophoresisThis protocol will make 2 liter of 50x solution. Adjust to your needs if necessary. This buffer will have only the dark blue than runs faster on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight.
Loading Buffer with GelRed for ElectrophoresisTo do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.
1 Kb Ladder with GelRed for ElectrophoresisUse the Fermentas GeneRuler 1kb ladder from the stock. Mix:
Good to go. Load 3 to 5 µl. |