Maloof Lab:loading dye: Difference between revisions
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<h3><font style="color:#C9D3EB;">6x Bromophenol Blue Loading Buffer for Electrophoresis</font></h3> | <h3><font style="color:#C9D3EB;">6x Bromophenol Blue Loading Buffer for Electrophoresis</font></h3> | ||
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This buffer will have only the <b>dark</b> blue than runs <b>faster</b> on the gel. | This buffer will have only the <b>dark</b> blue than runs <b>faster</b> on the gel. |
Revision as of 12:52, 16 February 2010
Room 2115 |
6x Xylene Cyanol Loading Buffer for ElectrophoresisThis buffer will have only the light blue than runs slower on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight. 6x Bromophenol Blue Loading Buffer for ElectrophoresisThis buffer will have only the dark blue than runs faster on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.
Dissolve well. It takes a while. An option is to shake at 37 degrees overnight.
Loading Buffer with GelRed for ElectrophoresisTo do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.
1 Kb Ladder with GelRed for ElectrophoresisUse the Fermentas GeneRuler 1kb ladder from the stock. Mix:
Good to go. Load 3 to 5 µl. |