Maloof Lab:miniprep reagents

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Recipes for miniprep solutions. For use with econospin miniprep protocol or miniprep without columns protocol Buffer 1 (Cell resuspension buffer) 50mM Tris 10mM EDTA 100 ug/mL RNase A For 100 mL 5 mL 1M Tris pH8 2 mL 0.5M EDTA 1 mL 10mg/mL RNase A 92 mL water Buffer 2 (Cell lysis solution) 0.2M NaOH 1% SDS For 100 mL 20 mL 1M NaOH 5 mL 20% SDS 75 mL water Buffer 3 (Neutralization Solution) 4M guanidine hydrochloride 0.5M sodium acetate For 100 mL 2.3g guanidine hydrochloride 4.9g potassium acetate pH to 4.2 with glacial acetic acid bring volume to 100 mL with water Wash buffer 60mM potassium acetate 8.3mM Tris-HCl (pH 7.5) 40μM EDTA (pH 8) 60% ethanol [[ For 100mL]] 1.2 mL 5M potassium acetate 0.83 mL 1M Tris (pH 7.5) 8 μL 0.5M EDTA 60 mL 100% ethanol 38mL water ADD 170 mL 95% ethanol to the wash buffer before first use!!