Maloof Lab:miniprep reagents

Buffer 1 (Cell resuspension buffer

50mM Tris 10mM EDTA 100 ug/mL RNase A

For 100 mL 5 mL 1M Tris pH8 2 mL 0.5M EDTA 1 mL 10mg/mL RNase A 92 mL water

Buffer 2 (Cell lysis solution

0.2M NaOH 1% SDA

For 100 mL 20 mL 1M NaOH 5 mL 20% SDS 75 mL water