Manual 2ab Assembly Digest

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(New page: Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix. <br> '''Lefty MasterMix''' (for 4uL miniprep DNA) *4uL water *1uL of NEB2 *1uL XhoI *1uL BamH1 '''Righty MasterMix'...)
Current revision (15:12, 11 June 2010) (view source)
 
Line 5: Line 5:
*1uL of NEB2
*1uL of NEB2
*1uL XhoI
*1uL XhoI
-
*1uL BamH1
+
*1uL ''BamH1''
'''Righty MasterMix''' (for 4uL miniprep DNA)
'''Righty MasterMix''' (for 4uL miniprep DNA)
Line 11: Line 11:
*1uL of NEB2
*1uL of NEB2
*1uL XhoI
*1uL XhoI
-
*1uL BglII <br>
+
*1uL ''BglII'' <br>
Incubate for 1 hour at 37deg C.
Incubate for 1 hour at 37deg C.

Current revision

Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix.

Lefty MasterMix (for 4uL miniprep DNA)

  • 4uL water
  • 1uL of NEB2
  • 1uL XhoI
  • 1uL BamH1

Righty MasterMix (for 4uL miniprep DNA)

  • 4uL water
  • 1uL of NEB2
  • 1uL XhoI
  • 1uL BglII

Incubate for 1 hour at 37deg C.

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