Manual 2ab Assembly Digest: Difference between revisions
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(New page: Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix. <br> '''Lefty MasterMix''' (for 4uL miniprep DNA) *4uL water *1uL of NEB2 *1uL XhoI *1uL BamH1 '''Righty MasterMix'...) |
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| Line 5: | Line 5: | ||
*1uL of NEB2 | *1uL of NEB2 | ||
*1uL XhoI | *1uL XhoI | ||
*1uL BamH1 | *1uL ''BamH1'' | ||
'''Righty MasterMix''' (for 4uL miniprep DNA) | '''Righty MasterMix''' (for 4uL miniprep DNA) | ||
| Line 11: | Line 11: | ||
*1uL of NEB2 | *1uL of NEB2 | ||
*1uL XhoI | *1uL XhoI | ||
*1uL BglII <br> | *1uL ''BglII'' <br> | ||
Incubate for 1 hour at 37deg C. | Incubate for 1 hour at 37deg C. | ||
Latest revision as of 12:12, 11 June 2010
Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix.
Lefty MasterMix (for 4uL miniprep DNA)
- 4uL water
- 1uL of NEB2
- 1uL XhoI
- 1uL BamH1
Righty MasterMix (for 4uL miniprep DNA)
- 4uL water
- 1uL of NEB2
- 1uL XhoI
- 1uL BglII
Incubate for 1 hour at 37deg C.
