Marek: Freeze-down/Thaw: Difference between revisions
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== | ==Overview== | ||
* | This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells). | ||
* | |||
==Materials== | |||
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate. | |||
*supply 1 (i.e. tubes of a certain size? spreaders?) | |||
*reagent 1 | |||
*X μL reagent 2 | |||
**component A (reagent 2 is made up of multiple components) | |||
**component B | |||
*equipment 1 | |||
*equipment 2 | |||
==Procedure== | |||
#Step 1 | |||
#Step 2 | |||
#*Step 2 has some additional information that goes with it. i.e. Keep at 4°C. | |||
#Step 3 | |||
##Step 3 has multiple sub-steps within it. | |||
##Enumerate each of those. | |||
==Notes== | |||
#List troubleshooting tips here. | |||
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites. | |||
#Anecdotal observations that might be of use to others can also be posted here. | |||
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | |||
==References== | |||
'''Relevant papers and books''' | |||
<!-- If this protocol has papers or books associated with it, list those references here. See the [[OpenWetWare:Biblio]] page for more information. --> | |||
<biblio> | |||
#Goldbeter-PNAS-1981 pmid=6947258 | |||
#Jacob-JMB-1961 pmid=13718526 | |||
#Ptashne-Genetic-Switch isbn=0879697164 | |||
</biblio> | |||
==Contact== | |||
*Who has experience with this protocol? | |||
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | |||
<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --> | |||
[[Category:Protocol]] | |||
[[Category:Needs attention]] <!--Delete this line once the protocol is complete--> | |||
<!-- Move the relevant categories above this line to tag your protocol with the label | |||
[[Category:In vitro]] | |||
[[Category:In vivo]] | |||
[[Category:In silico]] | |||
[[Category:DNA]] | |||
[[Category:RNA]] | |||
[[Category:Protein]] | |||
[[Category:Chemical]] | |||
[[Category:Escherichia coli]] | |||
[[Category:Yeast]] | |||
--> |
Revision as of 01:43, 18 May 2007
Overview
This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells).
Materials
List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.
- supply 1 (i.e. tubes of a certain size? spreaders?)
- reagent 1
- X μL reagent 2
- component A (reagent 2 is made up of multiple components)
- component B
- equipment 1
- equipment 2
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
- ISBN:0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.