Marie-Emmanuelle Kerros

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HOW TO ... MAKE PCR REAGENTS
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[[Category:User France]]
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Rutgers, October 2004
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1- 5x Tris Boric acid EDTA buffer
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For 2 liters:
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- TRIS base (Boehringer Mannheim 1814273): 108 g
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- Boric acid electrophoresis grade (FisherBiotech BP168-500): 55 g
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- 0.5 M EDTA (pH 8.0): 40 ml (or 9.3 g Na2EDTA)
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- adjust to pH 8.3
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- H2O to 2 liters
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2- EDTA 0.5 M pH = 8.0
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- EDTA: 18.61 g
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- NaOH: 2.0 g
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- H2O: 80 ml
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- dissolve on a magnetic stirrer
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- adjust to pH = 8.0 with NaOH 10N and 1N
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- H2O to 100 ml
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Current revision

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