Marie-Emmanuelle Kerros

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
HOW TO ... MAKE PCR REAGENTS
 
-
Rutgers, October 2004
 
-
 
-
 
-
1- 5x Tris Boric acid EDTA buffer
 
-
 
-
For 2 liters:
 
-
- TRIS base (Boehringer Mannheim 1814273): 108 g
 
-
- Boric acid electrophoresis grade (FisherBiotech BP168-500): 55 g
 
-
- 0.5 M EDTA (pH 8.0): 40 ml (or 9.3 g Na2EDTA)
 
-
- adjust to pH 8.3
 
-
- H2O to 2 liters
 
-
 
-
 
-
2- EDTA 0.5 M pH = 8.0
 
-
- EDTA: 18.61 g
 
-
- NaOH: 2.0 g
 
-
- H2O: 80 ml
 
-
- dissolve on a magnetic stirrer
 
-
- adjust to pH = 8.0 with NaOH 10N and 1N
 
-
- H2O to 100 ml
 

Revision as of 10:42, 11 September 2006

Personal tools