Marie-Emmanuelle Kerros: Difference between revisions

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-HOW TO ... MAKE PCR REAGENTS
-Rutgers, October 2004
-- 5x Tris Boric acid EDTA buffer
-For 2 liters:
-- TRIS base (Boehringer Mannheim 1814273):			108 g
-- Boric acid electrophoresis grade (FisherBiotech BP168-500):	55 g
-- 0.5 M EDTA (pH 8.0):													40 ml (or 9.3 g Na2EDTA)
-- adjust to pH 8.3
-- H2O to 2 liters
-- EDTA 0.5 M pH = 8.0
-- EDTA:	18.61 g
-- NaOH:	2.0 g
-- H2O:	80 ml
-- dissolve on a magnetic stirrer
-- adjust to pH = 8.0 with NaOH 10N and 1N
-- H2O to 100 ml

Revision as of 07:42, 11 September 2006