Materials

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Current revision (04:44, 25 June 2014) (view source)
(Undo revision 798541 by Alona Bozhchenko (Talk))
 
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<div style="padding: 2px">[[Image:OWWMaterials.png]]</div>
<div style="padding: 2px">[[Image:OWWMaterials.png]]</div>
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<div style="padding: 2px">'''Moving toward a better OWW Materials Page. Please [[Talk:Materials | visit the Materials Talk Page]] for details'''</div>
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{| cellspacing="1"
{| cellspacing="1"
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==Media==
==Media==
===Defined Media===
===Defined Media===
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* [[Neidhardt EZ Rich Defined]]  
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Defined Media]]-->
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* [[M9 salts]]
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{{#dpl:category=Material|category=Defined Media}}
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* [[M9 medium]] 
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* [[SC]]
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* [[SD]]
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===Standard Media===
===Standard Media===
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* [[LB]]  
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Standard Media]]-->
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* [[SOB]]
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{{#dpl:category=Material|category=Standard Media}}
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* [[SOC]]
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* [[T broth | T (Tryptone) Broth]]
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* [[Terrific Broth]]
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* [[YPD]]
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* [[Silver: YEPD Liquid]]
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* [[McKinney:7H9|7H9]] (for mycobacteria)
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===Cell Culture Media===
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* [[DMEM]]
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* [[RPMI]]
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* [[complete DMEM]]
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==Antibiotics==
==Antibiotics==
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{{#dpl:category=Material|category=Enzyme|category=Phosphatase}}
{{#dpl:category=Material|category=Enzyme|category=Phosphatase}}
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===DNA Polymerases===
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===[[DNA polymerase|DNA polymerases]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:DNA]]-->
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:DNA]]-->
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{{#dpl:category=Material|category=Enzyme|category=Polymerase|category=DNA}}
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{{#dpl:category=Material|category=Enzyme|category=Polymerase|category=DNA|nottitlematch=DNA polymerase}}
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===[[DNA ligase | DNA ligases]]===
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Ligase]] and [[Category:DNA]]-->
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{{#dpl:category=Material|category=Enzyme|category=Ligase|category=DNA|nottitlematch=DNA ligase}}
===RNA Polymerases===
===RNA Polymerases===
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Endonuclease]]-->
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Endonuclease]]-->
{{#dpl:category=Material|category=Enzyme|category=Endonuclease|nottitlematch=Restriction enzymes}}
{{#dpl:category=Material|category=Enzyme|category=Endonuclease|nottitlematch=Restriction enzymes}}
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===Other===
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Other]]-->
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{{#dpl:category=Material|category=Enzyme|category=Other}}
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==Proteases==
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Proteases]]-->
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{{#dpl:category=Material|category=Enzyme|category=Proteases}}
|rowspan="2" width="152px" class="green1"|
|rowspan="2" width="152px" class="green1"|
==Buffers==
==Buffers==
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* [[ACK lysis buffer]]
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Buffers]]-->
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* [[B-Per II]] - lysis buffer
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{{#dpl:category=Material|category=Buffers}}
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* [[HEPES]]
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* [[MES]]
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* [[NEB buffers]]
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* [[PIPES]]
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* [[PBS]]
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* [[SDS sample buffer]]
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* [[SSC buffer]]
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* [[TB buffer]]
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* [[TE buffer]]
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* [[Tris]]
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* [[TSS]]
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* [[Phosphate buffer]]
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==Acids and bases==
==Acids and bases==
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* [[Acid concentration chart]]
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Acids and bases]]-->
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* [[5-Fluoroorotic acid]]
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{{#dpl:category=Material|category=Acids and bases}}
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* [[Sodium hydroxide]]
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==Other common chemicals==
==Other common chemicals==
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* [[aTc]]
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Chemical]]-->
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* [[3-Aminotriazole]]
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{{#dpl:category=Material|category=Chemical}}
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* [[Arabinose]]  
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* [[DEPC]]
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* [[DMPC]]
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* [[EDTA]]
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* [[Glucose]]
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* [[IPTG]]
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* [[ONPF]]
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* [[Phenol]]
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* [[Raffinose]]
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* [[Spermine]]
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==Fixatives==
==Fixatives==
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* [[polymethanal (paraformaldehyde)]]
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Fixatives]]-->
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* [[glutaraldehyde]]
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{{#dpl:category=Material|category=Fixatives}}
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==Reporters==
==Reporters==
===[[Dyes and stains]]===
===[[Dyes and stains]]===
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* [[7-AAD]]
* [[Acridine Orange]]  
* [[Acridine Orange]]  
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* [[Annexin-V]]
* [[Carboxyfluorescein Diacetate]]  
* [[Carboxyfluorescein Diacetate]]  
* [[DAPI]]  
* [[DAPI]]  
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===[[Fluorescent proteins]]===
===[[Fluorescent proteins]]===
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* [[CFP]]  
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Protein]] and [[Category:Reporter]]-->
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* [[Emerald|Emerald-GFP]]
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* [[GFP]]  
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{{#dpl:category=Protein|category=Reporter|category=Material}}
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* [[mRFP1]]
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* [[YFP]]
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===Enzymes===
===Enzymes===
{{#dpl:category=Enzyme|category=Reporter|category=Material}}
{{#dpl:category=Enzyme|category=Reporter|category=Material}}
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==Detergents==
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{{#dpl:category=Detergents|category=Material}}
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|-
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|width="210px" class="green2"|
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==Electrophoresis==
==Electrophoresis==
===Agarose gel electrophoresis===
===Agarose gel electrophoresis===
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* [[1X TAE]]
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Agarose gel electrophoresis]]-->
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* [[10X TBE]]  
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{{#dpl:category=Material|category=Agarose gel electrophoresis}}
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* [[Agarose gel loading buffer]]
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* [[DNA ladder]]
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* [[Ethidium Bromide]]
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* [[Sybr Safe]]
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===PAGE===
===PAGE===
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* [[SDS-PAGE]]
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:PAGE]]-->
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* [[Destain]]  
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{{#dpl:category=Material|category=PAGE}}
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* [[Coomassie stain]]  
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* [[PLS]]
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|width="200px" class="green1"|
|width="200px" class="green1"|
==Miscellaneous==
==Miscellaneous==
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* [[dNTPs]]  
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<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Miscellaneous]]-->
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* [[ProteinA_Sepharose Beads]]
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{{#dpl:category=Material|category=Miscellaneous}}
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*[[Materials/Search|'''Search materials by category''']]
*[[Materials/Search|'''Search materials by category''']]
*[[Materials/Template|'''Add a new material page''']]
*[[Materials/Template|'''Add a new material page''']]
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*[[Materials/Additional Categories Needed |'''Materials In Need of Better Categorization''']]
*'''Get help at [[Questions and Answers|OWW Q&A]]'''
*'''Get help at [[Questions and Answers|OWW Q&A]]'''
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</showhide>
</showhide>
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__NOTOC__
__NOTOC__
</div>
</div>

Current revision


Image:OWWMaterials.png



Moving toward a better OWW Materials Page. Please visit the Materials Talk Page for details


Media

Defined Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

RNA Polymerases

Restriction enzymes

Other

Proteases

Buffers

Acids and bases

Other common chemicals

Fixatives


Reporters

Dyes and stains

Fluorescent proteins

Enzymes


Detergents



Electrophoresis

Agarose gel electrophoresis

PAGE


Miscellaneous




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