Materials: Difference between revisions

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== Media ==
<div id="mainpage">
=== Defined Media ===
 
# [[M9 salts]]  
<hr class="divider">
# [[M9 media]]  
<div style="padding: 2px">[[Image:OWWMaterials.png]]</div>
# [[M9 supplemented media]]
<hr class="divider">
# [[SD]]
 
=== Standard Media ===
 
# [[LB]]  
 
# [[T Broth]]
<hr class="divider">
#[[YPD]]
 
<div style="padding: 2px">'''Moving toward a better OWW Materials Page. Please [[Talk:Materials | visit the Materials Talk Page]] for details'''</div>
 
<hr class="divider">
 
 
 
{| cellspacing="1"
|- valign="top"
|rowspan="2" width="200px" class="green1"|
==Media==
===Defined Media===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Defined Media]]-->
{{#dpl:category=Material|category=Defined Media}}
 
===Standard Media===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Standard Media]]-->
{{#dpl:category=Material|category=Standard Media}}


==Antibiotics==
==Antibiotics==
#[[Ampicillin]]
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Antibiotic]]-->
#[[G418]]
{{#dpl:category=Material|category=Antibiotic}}
#[[Kanamycin]]
 
#[[Phleomycin]]
|width="210px" class="green3"|
#[[Rifampicin]]
 
#[[Streptomycin]]
==Enzymes==
#[[Tetracycline]]
===Phosphatases===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Phosphatase]]-->
{{#dpl:category=Material|category=Enzyme|category=Phosphatase}}
 
===[[DNA polymerase|DNA polymerases]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:DNA]]-->
{{#dpl:category=Material|category=Enzyme|category=Polymerase|category=DNA|nottitlematch=DNA polymerase}}
 
===[[DNA ligase | DNA ligases]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Ligase]] and [[Category:DNA]]-->
{{#dpl:category=Material|category=Enzyme|category=Ligase|category=DNA|nottitlematch=DNA ligase}}
 
===RNA Polymerases===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:RNA]]-->
{{#dpl:category=Material|category=Enzyme|category=Polymerase|category=RNA}}
 
===[[Restriction enzymes]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Endonuclease]]-->
{{#dpl:category=Material|category=Enzyme|category=Endonuclease|nottitlematch=Restriction enzymes}}
 
===Other===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Other]]-->
{{#dpl:category=Material|category=Enzyme|category=Other}}
 
==Proteases==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Proteases]]-->
{{#dpl:category=Material|category=Enzyme|category=Proteases}}
 
|rowspan="2" width="152px" class="green1"|
 
==Buffers==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Buffers]]-->
{{#dpl:category=Material|category=Buffers}}
 
==Acids and bases==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Acids and bases]]-->
{{#dpl:category=Material|category=Acids and bases}}
 
==Other common chemicals==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Chemical]]-->
{{#dpl:category=Material|category=Chemical}}
 
==Fixatives==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Fixatives]]-->
{{#dpl:category=Material|category=Fixatives}}
 
 
|width="204px" class="green2"|
 
==Reporters==
===[[Dyes and stains]]===
* [[7-AAD]]
* [[Acridine Orange]]
* [[Annexin-V]]
* [[Carboxyfluorescein Diacetate]]
* [[DAPI]]
* [[Fluorescein diacetate]]
* [[Hoechst 33342]]
* [[Propidium iodide]]
* [[SYBR Gold]]
* [[SYBR Green I]]
* [[SYBR Green II]]
* [[Sybr Safe]]
* [[SYTO]]
 
===[[Fluorescent proteins]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Protein]] and [[Category:Reporter]]-->
 
{{#dpl:category=Protein|category=Reporter|category=Material}}
 
===Enzymes===
{{#dpl:category=Enzyme|category=Reporter|category=Material}}
 
 
==Detergents==
{{#dpl:category=Detergents|category=Material}}
 
 
|-
 
|width="210px" class="green2"|
 
 
==Electrophoresis==
===Agarose gel electrophoresis===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Agarose gel electrophoresis]]-->
{{#dpl:category=Material|category=Agarose gel electrophoresis}}


== Enzymes ==
===PAGE===
=== Phosphatases ===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:PAGE]]-->
# [[Antarctic Phosphatase]]
{{#dpl:category=Material|category=PAGE}}
# [[Alkaline Phosphatase]]


=== RNA Polymerases ===
# [[T7 RNA Polymerase]]
# [[T4 RNA Polymerase]]


=== DNA Polymerases ===
|width="200px" class="green1"|
# [[Vent]]
# [[Taq]]


== Reporters ==
==Miscellaneous==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Miscellaneous]]-->
{{#dpl:category=Material|category=Miscellaneous}}


=== [[Dyes and stains]] ===


====Useful links====
http://www.probes.com/handbook/sections/0801.html<br>
http://www.probes.com/handbook/sections/1502.html


====Individual dyes====
# [[Acridine Orange]]
# [[Carboxyfluorescein Diacetate]]
# [[DAPI]]
# [[Fluorescein diacetate]]
# [[Hoechst 33342]]
# [[Propidium iodide]]
# [[SYBR Green I]]
# [[SYBR Green II]]
# [[SYTO]]


====Notes====
*[[Materials/Search|'''Search materials by category''']]
There don't appear to be any dyes that selectively stain live cells.  When people want to detect live cells, I think that what they do is the following:  "The principle of this approach is to use simultaneously a permeant (SYBR Green [more recently SYTO green]; Molecular Probes) and an impermeant (propidium iodide) probe and to take advantage of the energy transfer which occurs between them when both probes are staining nucleic acids. A full quenching of the permeant probe fluorescence by the impermeant probe will point to cells with a compromised membrane, a partial quenching will indicate cells with a slightly damaged membrane, and a lack of quenching will characterize intact membrane cells identified as viable."  From Applied and Environmental Microbiology, October 2001, p. 4662-4670, Vol. 67, No. 10.  So most stains that people bill as live cell stains are only membrane permeant stains and therefore stain both live and dead cells.
*[[Materials/Template|'''Add a new material page''']]
*[[Materials/Additional Categories Needed |'''Materials In Need of Better Categorization''']]
*'''Get help at [[Questions and Answers|OWW Q&A]]'''
|}


== Agarose gels ==
<!-- No show/hide on page
<showhide>
__SHOWALL__ __HIDEALL__
</showhide>
-->


#[[1X TAE]]
__NOTOC__
#[[Loading dye]]
</div>
#[[DNA ladder]]
#[[EtBr | Ethidium bromide]]

Revision as of 01:44, 25 June 2014


Moving toward a better OWW Materials Page. Please visit the Materials Talk Page for details


Media

Defined Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

RNA Polymerases

Restriction enzymes

Other

Proteases

Buffers

Acids and bases

Other common chemicals

Fixatives

Reporters

Dyes and stains

Fluorescent proteins

Enzymes


Detergents



Electrophoresis

Agarose gel electrophoresis

PAGE

Miscellaneous




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