Materials: Difference between revisions

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<div style="padding: 2px">[[Image:OWWMaterials.png]]</div>
<div style="padding: 2px">[[Image:OWWMaterials.png]]</div>
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<div style="padding: 2px">'''Moving toward a better OWW Materials Page. Please [[Talk:Materials | visit the Materials Talk Page]] for details'''</div>
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==Media==
==Media==
===Defined Media===
===Defined Media===
* [[Neidhardt EZ Rich Defined]]  
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Defined Media]]-->
* [[M9 salts]]
{{#dpl:category=Material|category=Defined Media}}
* [[M9 media]] 
* [[SC]]
* [[SD]]


===Standard Media===
===Standard Media===
* [[LB]]  
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Standard Media]]-->
* [[SOB]]
{{#dpl:category=Material|category=Standard Media}}
* [[SOC]]
* [[T broth | T (Tryptone) Broth]]
* [[Terrific Broth]]
* [[YPD]]
* [[Silver: YEPD Liquid]]


==Buffers==
==Antibiotics==
* [[HEPES]]
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Antibiotic]]-->
* [[NEB buffers]]
{{#dpl:category=Material|category=Antibiotic}}
* [[PIPES]]
* [[10x PBS]]
* [[TB buffer]]
* [[TE buffer]]
* [[Tris]]
* [[TSS]]


==Acids and Bases==
|width="210px" class="green3"|
* [[Acid concentration chart]]


==Enzymes==
===Phosphatases===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Phosphatase]]-->
{{#dpl:category=Material|category=Enzyme|category=Phosphatase}}


===[[DNA polymerase|DNA polymerases]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:DNA]]-->
{{#dpl:category=Material|category=Enzyme|category=Polymerase|category=DNA|nottitlematch=DNA polymerase}}


===[[DNA ligase | DNA ligases]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Ligase]] and [[Category:DNA]]-->
{{#dpl:category=Material|category=Enzyme|category=Ligase|category=DNA|nottitlematch=DNA ligase}}


===RNA Polymerases===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:RNA]]-->
{{#dpl:category=Material|category=Enzyme|category=Polymerase|category=RNA}}


===[[Restriction enzymes]]===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Endonuclease]]-->
{{#dpl:category=Material|category=Enzyme|category=Endonuclease|nottitlematch=Restriction enzymes}}


===Other===
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Other]]-->
{{#dpl:category=Material|category=Enzyme|category=Other}}


==Proteases==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Proteases]]-->
{{#dpl:category=Material|category=Enzyme|category=Proteases}}


|rowspan="2" width="152px" class="green1"|


==Buffers==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Buffers]]-->
{{#dpl:category=Material|category=Buffers}}


[[Materials/Template|'''Add a new material''']]
==Acids and bases==
|width="210px" class="green3"|
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Acids and bases]]-->
 
{{#dpl:category=Material|category=Acids and bases}}
==Enzymes==
===Phosphatases===
* [[Antarctic Phosphatase]]  
* [[Alkaline Phosphatase]]  
===RNA Polymerases===
* [[T7 RNA Polymerase]]
* [[T4 RNA Polymerase]]
===DNA Polymerases===
* [[Vent]]
* [[Taq]]
* [[Terminal Transferase]]
* [[Phusion]]
* [[Reverse Transcriptase]]


===Restriction Enzymes===
==Other common chemicals==
* [[Restriction enzymes]]
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Chemical]]-->
{{#dpl:category=Material|category=Chemical}}


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==Fixatives==
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Fixatives]]-->
{{#dpl:category=Material|category=Fixatives}}


==Antibiotics==
* [[Ampicillin]]
* [[Apramycin]]
* [[Carbenicillin]]
* [[Chloramphenicol]]
* [[Erythromycin]]
* [[G418]]
* [[Gentamycin]]
* [[Hygromycin]]
* [[Kanamycin]]
* [[Nalidixic Acid]]
* [[Neomycin]]
* [[Phleomycin]]
* [[Puromycin]]
* [[Rifampicin]]
* [[Spectinomycin]]
* [[Streptomycin]]
* [[Tetracycline]]
* [[Trimethoprim]]


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==Reporters==
==Reporters==
===[[Dyes and stains]]===
===[[Dyes and stains]]===
* [[7-AAD]]
* [[Acridine Orange]]  
* [[Acridine Orange]]  
* [[Annexin-V]]
* [[Carboxyfluorescein Diacetate]]  
* [[Carboxyfluorescein Diacetate]]  
* [[DAPI]]  
* [[DAPI]]  
* [[Fluorescein diacetate]]  
* [[Fluorescein diacetate]]  
* [[Hoechst 33342]]  
* [[Hoechst 33342]]  
* [[Propidium iodide]]  
* [[Propidium iodide]]
* [[SYBR Gold]]
* [[SYBR Green I]]  
* [[SYBR Green I]]  
* [[SYBR Green II]]  
* [[SYBR Green II]]
* [[SYTO]]  
* [[Sybr Safe]]
* [[SYTO]]
 
===[[Fluorescent proteins]]===
===[[Fluorescent proteins]]===
* [[CFP]]  
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Protein]] and [[Category:Reporter]]-->
* [[Emerald|Emerald-GFP]]
 
* [[GFP]]  
{{#dpl:category=Protein|category=Reporter|category=Material}}
* [[mRFP1]]
 
* [[YFP]]
===Enzymes===
{{#dpl:category=Enzyme|category=Reporter|category=Material}}
 
 
==Detergents==
{{#dpl:category=Detergents|category=Material}}


===Misc===
* [[Beta-lactamase]]


|-
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==Electrophoresis==
==Electrophoresis==
===Agarose gel electrophoresis===
===Agarose gel electrophoresis===
* [[1X TAE]]
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Agarose gel electrophoresis]]-->
* [[10X TBE]]
{{#dpl:category=Material|category=Agarose gel electrophoresis}}
* [[Loading dye]]  
 
* [[DNA ladder]]
* [[Ethidium Bromide]]
* [[Sybr Safe]]
===PAGE===
===PAGE===
* [[Destain]]  
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:PAGE]]-->
* [[Coomassie stain]]  
{{#dpl:category=Material|category=PAGE}}
* [[PLS]]
 
 
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==Miscellaneous==
==Miscellaneous==
* [[aTc]]
<!--To list a material here, "tag" the protocol page with the [[Category:Material]] and [[Category:Miscellaneous]]-->
* [[Arabinose]]
{{#dpl:category=Material|category=Miscellaneous}}
* [[B-Per II]] - lysis buffer
* [[dNTPs]]  
* [[EDTA]]
* [[IPTG]]
* [[ONPF]]
* [[Phenol]]
* [[ProteinA_Sepharose Beads]]
* [[Raffinose]]
* [[3-Aminotriazole]]
* [[5-Fluoroorotic acid]]


*[[Materials/Search|'''Search materials by category''']]
*[[Materials/Template|'''Add a new material page''']]
*[[Materials/Additional Categories Needed |'''Materials In Need of Better Categorization''']]
*'''Get help at [[Questions and Answers|OWW Q&A]]'''
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Revision as of 01:44, 25 June 2014


Moving toward a better OWW Materials Page. Please visit the Materials Talk Page for details


Media

Defined Media

Standard Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

RNA Polymerases

Restriction enzymes

Other

Proteases

Buffers

Acids and bases

Other common chemicals

Fixatives

Reporters

Dyes and stains

Fluorescent proteins

Enzymes


Detergents



Electrophoresis

Agarose gel electrophoresis

PAGE

Miscellaneous




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