Matt Gethers: Difference between revisions

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I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the [[Endy Lab]] at [http://mit.edu MIT]. I will be documenting my UROP projects [[Endy:Translation demand | here]]. I will put my 20.109 work directly below.
I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the [[Endy Lab]] at [http://mit.edu MIT]. I have links to my UROP projects and 20.109 work directly below.


==UROP Projects==
[[Endy:Translation demand | Translation Demand]]
[http://bcanton.private.openwetware.org/wiki/Lab_Notebook/Matt/Mitochondria_Project Mitochondrial Engineering]
==20.109 Work==
==20.109 Work==


Revision as of 06:30, 20 July 2007

I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I have links to my UROP projects and 20.109 work directly below.


UROP Projects

Translation Demand Mitochondrial Engineering

20.109 Work

Reagents

List of Needed Reagents:

  1. Tris.SO4
  2. Dithiothreitol
  3. Sorbitol
  4. KPi
  5. Zymolyase 5000
  6. Mannitol
  7. Tris-HCl
  8. Phenylmethylsulfonyl fluoride (PMSF)
  9. PBS
  10. Beta Mercaptoethanol
  11. Reticulocyte Lysate
  12. Hepes/KOH
  13. ATP
  14. MgCl2
  15. Phosphoenolpyruvate (PEP)
  16. Pyruvate kinase
  17. GTP
  18. PEG 3500
  19. LiAc
  20. Carrier DNA (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626)
  21. EDTA
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