Matt Gethers

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(M13 Engineering Ideas)
(M13 Engineering Ideas)
Line 13: Line 13:
| II||Extract gene X  
| II||Extract gene X  
|-
|-
-
| V||
+
| V|| Add some base pairs between V and VII to allow for a restriction site
|-
|-
| VII||Separate from gene IX  
| VII||Separate from gene IX  

Revision as of 00:36, 14 February 2007

I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

M13 Engineering Ideas

Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences. In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like Biobricks to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.
Gene Modification
XExtract from gene II
IIExtract gene X
V Add some base pairs between V and VII to allow for a restriction site
VIISeparate from gene IX
IIIChange the GTG to ATG Start?
VI
ISeparate from genes IV and XI
XISeparate from genes I and IV
IVSeparate from genes I and XI
M13 ORI 1 & 2 Are two ORIs necessary? If so, can they be consolidated?
KanR
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