Matt Gethers: Difference between revisions
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*[[User:Mgethers/Research Proposal|Research Proposal]] | *[[User:Mgethers/Research Proposal|Research Proposal]] | ||
==Reagents== | |||
List of Needed Reagents: | |||
#Tris.SO<sub>4</sub> | |||
#Dithiothreitol | |||
#Sorbitol | |||
#KP<sub>i</sub> | |||
#Zymolyase 5000 | |||
#Mannitol | |||
#Tris-HCl | |||
#Phenylmethylsulfonyl fluoride (PMSF) | |||
#PBS | |||
#Beta Mercaptoethanol | |||
#Reticulocyte Lysate | |||
#Hepes/KOH | |||
#ATP | |||
#MgCl<sub>2</sub> | |||
#Phosphoenolpyruvate (PEP) | |||
#Pyruvate kinase | |||
#GTP | |||
#PEG 3500 | |||
#LiAc | |||
#Carrier DNA (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626) | |||
#EDTA |
Revision as of 11:06, 2 July 2007
I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.
20.109 Work
Reagents
List of Needed Reagents:
- Tris.SO4
- Dithiothreitol
- Sorbitol
- KPi
- Zymolyase 5000
- Mannitol
- Tris-HCl
- Phenylmethylsulfonyl fluoride (PMSF)
- PBS
- Beta Mercaptoethanol
- Reticulocyte Lysate
- Hepes/KOH
- ATP
- MgCl2
- Phosphoenolpyruvate (PEP)
- Pyruvate kinase
- GTP
- PEG 3500
- LiAc
- Carrier DNA (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626)
- EDTA