Matt Gethers

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I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

Reagents

List of Needed Reagents:

  1. Tris.SO4
  2. Dithiothreitol
  3. Sorbitol
  4. KPi
  5. Zymolyase 5000
  6. Mannitol
  7. Tris-HCl
  8. Phenylmethylsulfonyl fluoride (PMSF)
  9. PBS
  10. Beta Mercaptoethanol
  11. Reticulocyte Lysate
  12. Hepes/KOH
  13. ATP
  14. MgCl2
  15. Phosphoenolpyruvate (PEP)
  16. Pyruvate kinase
  17. GTP
  18. PEG 3500
  19. LiAc
  20. Carrier DNA (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626)
  21. EDTA
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