Matt Gethers
From OpenWetWare
I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.
20.109 Work
Reagents
List of Needed Reagents:
- Tris.SO4
- Dithiothreitol
- Sorbitol
- KPi
- Zymolyase 5000
- Mannitol
- Tris-HCl
- Phenylmethylsulfonyl fluoride (PMSF)
- PBS
- Beta Mercaptoethanol
- Reticulocyte Lysate
- Hepes/KOH
- ATP
- MgCl2
- Phosphoenolpyruvate (PEP)
- Pyruvate kinase
- GTP
- PEG 3500
- LiAc
- Carrier DNA (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626)
- EDTA