M13 Engineering Ideas
|X||Extract from gene II|
|II||Extract gene X|
|VII||Separate from gene IX|
|III||Change the GTG to ATG Start?|
|I||Separate from genes IV and XI|
|XI||Separate from genes I and IV|
|IV||Separate from genes I and XI|
|M13 ORI 1|
|M13 ORI 2||
Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences.
In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like [[ ||Biobricks]] to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.