Matt Gethers

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I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

M13 Engineering Ideas

Gene Modification
XExtract from gene II
IIExtract gene X
V
VIISeparate from gene IX
IIIChange the GTG to ATG Start?
VI
ISeparate from genes IV and XI
XISeparate from genes I and IV
IVSeparate from genes I and XI
M13 ORI 1
KanR
M13 ORI 2

Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences.

In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like [[ ||Biobricks]] to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.

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