Matt Gethers

From OpenWetWare

Revision as of 00:40, 14 February 2007 by Mgethers (Talk | contribs)
Jump to: navigation, search

I'm currently a sophomore in course 20. When I'm behaving myself, I'm allowed to work in the Endy Lab at MIT. I will be documenting my UROP projects here. I will put my 20.109 work directly below.

20.109 Work

M13 Engineering Ideas

Extractions, separations, etc. include ensuring that each gene has its own promoter, RBS, terminator, and any other pertinent regulatory sequences. In addition to separating the genes, it would also be wise to develop or utilize/modify an existing system like Biobricks to build the genome so that convenient restriction sites exist between the genes so they can be easily removed or replaced.
Gene Modification
XExtract from gene II.
IIExtract gene X.
V Add some base pairs between V and VII to allow for a restriction site.
VIISeparate from gene IX.
IIIChange the GTG to ATG Start?
VI Add some base pairs between III and VI and VI anda I to allow for restriction sites.
ISeparate from genes IV and XI.
XISeparate from genes I and IV.
IVSeparate from genes I and XI.
M13 ORI 1 & 2 Are two ORIs necessary? If so, can they be consolidated?
KanR Possible to remove bps 6600 - 7100 upstream of KanR? Does this DNA have functional significance?
Personal tools