Matt Gethers/20.380 HIV Project/Meeting Notes/3.4.08 (Final Cut on Design Ideas): Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 7: | Line 7: | ||
===Make list of issues/questions to consider in implementing the engineered RBC idea.=== | ===Make list of issues/questions to consider in implementing the engineered RBC idea.=== | ||
====Questions==== | ====Questions==== | ||
*What do we need to put into the person? What stage of progenitor cells? | *What do we need to put into the person? What stage of progenitor cells? - '''(done!)''' | ||
*Immunity issues with progenitor cells? | *Immunity issues with progenitor cells? - '''Rob''' | ||
*How are RBCs removed/recycled from body? | *How are RBCs removed/recycled from body? - '''Courtney''' | ||
*Can HIV infect progenitor cells? | *Can HIV infect progenitor cells? - '''Matt''' | ||
*How to couple CD4 expression, etc with RBC differentiation? | *How to couple CD4 expression, etc with RBC differentiation? - '''Rob''' | ||
*Malaria | *Malaria - '''Rob''' | ||
*What's necessary and sufficient for HIV infection? | *What's necessary and sufficient for HIV infection? - '''Matt''' | ||
*What to do with HIV once in cell? Do we need it to do anything? | *What to do with HIV once in cell? Do we need it to do anything? - '''Rob''' | ||
*How to prevent HIV from leaving RBC? | *How to prevent HIV from leaving RBC? - '''Jessie''' | ||
*Death switch? - '''Jessie''' | |||
*Death switch? | *Exploding RBCs? - '''David''' | ||
*Exploding RBCs? | *Any natural conditions where RBCs die or explode? - '''David''' | ||
*Any natural conditions where RBCs die or explode? | *Take out hemoglobin? Anything else to knock out? - '''Yi''' | ||
*Take out hemoglobin? Anything else to knock out? | *Pathologies of RBCs - esp. sickle cell anemia, thalassemia - '''Yi''' | ||
*Pathologies of RBCs - esp. sickle cell anemia | *Ideal concentration of RBC progenitors? - '''Steph''' | ||
*Ideal concentration of RBC progenitors? | *How to get into bone marrow? Easy injection method? - '''Steph''' | ||
*How to get into bone marrow? Easy injection method? | *Role of progenitors? - '''Steph''' | ||
===Make list of issues/questions to consider in implementing the superinfection idea.=== | ===Make list of issues/questions to consider in implementing the superinfection idea.=== | ||
Line 30: | Line 29: | ||
*Does superinfection apply across strains? | *Does superinfection apply across strains? | ||
*Fidelity of superinfection? How often does it fail? | *Fidelity of superinfection? How often does it fail? | ||
- '''Matt''' | |||
#Divide work for presentation next week (3/12/09) | #Divide work for presentation next week (3/12/09) |
Revision as of 18:09, 10 March 2009
3.4.08 (Final Cut on Design Ideas)
To Do
Make list of issues/questions to consider in implementing the engineered T-Cell idea.
Make list of issues/questions to consider in implementing the engineered RBC idea.
Questions
- What do we need to put into the person? What stage of progenitor cells? - (done!)
- Immunity issues with progenitor cells? - Rob
- How are RBCs removed/recycled from body? - Courtney
- Can HIV infect progenitor cells? - Matt
- How to couple CD4 expression, etc with RBC differentiation? - Rob
- Malaria - Rob
- What's necessary and sufficient for HIV infection? - Matt
- What to do with HIV once in cell? Do we need it to do anything? - Rob
- How to prevent HIV from leaving RBC? - Jessie
- Death switch? - Jessie
- Exploding RBCs? - David
- Any natural conditions where RBCs die or explode? - David
- Take out hemoglobin? Anything else to knock out? - Yi
- Pathologies of RBCs - esp. sickle cell anemia, thalassemia - Yi
- Ideal concentration of RBC progenitors? - Steph
- How to get into bone marrow? Easy injection method? - Steph
- Role of progenitors? - Steph
Make list of issues/questions to consider in implementing the superinfection idea.
Questions
- Does superinfection apply across strains?
- Fidelity of superinfection? How often does it fail?
- Matt
- Divide work for presentation next week (3/12/09)
- Modify calendar to reflect changes in schedule.
Minutes
Follow Up
Erythropoiesis
- Cell Lineage Map
- Need to start with hematapoeic stem cell
- knock out other lineages? (maybe we could leave them? would it be a problem?)
- Need to start with hematapoeic stem cell