Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Supermix Template

From OpenWetWare

Revision as of 21:43, 8 July 2008 by Mgethers (talk | contribs) (→‎PCR Supermix Template)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to navigation Jump to search

PCR Supermix Template

Rxn Mix

Reagent	Volume/Amount
DNA Template	1.0 μl from Colony Suspension
	1.2 μl 1 μM Stock
	1.2 μl 1 μM Stock
2x PCR Master	7.5 μl
H₂0	4.1 μl
Total	15 μl

Rxn Conditions

Annealing Temperature: ^oC

Extension Time:

Cycle (Taq)

Step	Temperature	Duration	Notes
Initial Denaturation	95^oC	2 minutes
Repeat Cyclic Steps 35x
Cyclic Denaturation	94^oC	30 Seconds
Cyclic Annealing	^oC	30 Seconds
Cyclic Extension	72^oC	minute
Repeat Cyclic Steps 35x
Final Extension	72^oC	10 minutes

Run Notes

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Matt_Gethers/CRI,_Thailand/Labwork/PCRs/PCR_Supermix_Template&oldid=219460"

Navigation menu