McClean:BlueLightSetup: Difference between revisions
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==Overview== | |||
This protocol describes how to connect blue LEDs for cells which need to be incubated in blue light. | |||
==Materials== | |||
* Blue LED [http://www.luxeonstar.com/blue-470nm-sinkpad-ii-25mm-star-o-led-70lm] | |||
* XITANIUM 12W 350mA Converter [http://www.brite-lite.com/Products/led120a0350c33f.html] | |||
* Connector [http://www.digikey.com/product-detail/en/2058943-1/A100192-ND/2136136] | |||
* Any 120W power cable, with the end opposite from the power plug stipped. | |||
** OPTIONAL: Any circuit breadboard | |||
** Connecting wires for the breadboard. | |||
==Stock Solutions== | |||
'''Stock Solution 1''' | |||
* This is a very simple solution, so we only need a one line description of how to make it. | |||
'''Stock Solution 2''' | |||
This is a more involved solution, so we will describe how to make it in several steps: | |||
# Step 1 | |||
# Step 2 | |||
# Step 3 | |||
==Protocol== | |||
# Step 1 | |||
# Step 2 | |||
# Step 3 | |||
==Notes== | |||
<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!--> | |||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | |||
#List troubleshooting tips here. | |||
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites. | |||
#Anecdotal observations that might be of use to others can also be posted here. | |||
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | |||
==References== | |||
Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS | |||
CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96. | |||
==Contact== | |||
<!--Change the information below to your info if you add a new protocol--> | |||
*'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)''' | |||
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | |||
<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --> | |||
[[Category:Protocol]] | |||
<!-- Move the relevant categories above this line to tag your protocol with the label | |||
[[Category:Protocol]] | |||
[[Category:Media]] | |||
[[Category:Yeast]] | |||
--> |
Revision as of 12:42, 1 July 2015
Overview
This protocol describes how to connect blue LEDs for cells which need to be incubated in blue light.
Materials
- Blue LED [1]
- XITANIUM 12W 350mA Converter [2]
- Connector [3]
- Any 120W power cable, with the end opposite from the power plug stipped.
- OPTIONAL: Any circuit breadboard
- Connecting wires for the breadboard.
Stock Solutions
Stock Solution 1
- This is a very simple solution, so we only need a one line description of how to make it.
Stock Solution 2
This is a more involved solution, so we will describe how to make it in several steps:
- Step 1
- Step 2
- Step 3
Protocol
- Step 1
- Step 2
- Step 3
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.
Contact
- Megan N McClean 14:01, 20 July 2011 (EDT)
or instead, discuss this protocol.