McClean:BlueLightSetup: Difference between revisions

Revision as of 13:14, 1 July 2015

This protocol describes how to connect blue LEDs for cells which need to be incubated in blue light.

Luxeon Blue LED. The light shown below is no longer produced. This is similar. [1]
XITANIUM 12W 350mA Converter [2]
TE Connectivity AMP Connectors 2058943-1 [3]
Any 120W power cable, with the end opposite from the power plug stipped.
Electrical Tape
Solder and Solder wire
OPTIONAL: To connect multiple Blue Light LEDs to one power source:
- Any circuit breadboard
- Connecting wires for the breadboard.

Step 1 Connect Power Plug to XITANIUM converter. The converter has two small pins which can be pushed down (shown by my pen in the photo). Push the pin down. Insert the stripped cable wire. Release pin. The cable wire should resist being pulled out if this is done properly. Repeat for both wires and corresponding pins. Wrap in electrical tape.
Step 2 Solder Connecting wire to the sides of the LED.
Step 3 Insert the connecting wire to the XITANIUM converter. Plug it in.

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

List troubleshooting tips here.
You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.

@@ Line 18: / Line 18: @@
 ==Simple Protocol==
-# Step 1 Connect Power Plug to XITANIUM converter. The converter has two small pins which can be pushed down (shown by my pen in the photo). Push the pin down. Insert the stripped cable wire. Release pin. The cable wire should resist being pulled out if this is done properly. Repeat for both wires and corresponding pins. Wrap in electrical tape.
+* Step 1 Connect Power Plug to XITANIUM converter. The converter has two small pins which can be pushed down (shown by my pen in the photo). Push the pin down. Insert the stripped cable wire. Release pin. The cable wire should resist being pulled out if this is done properly. Repeat for both wires and corresponding pins. Wrap in electrical tape.
 **[[Image:WiresInXITANIUM.jpg|||100px|||]]
-# Step 2 Solder Connecting wire to the sides of the LED.
+* Step 2 Solder Connecting wire to the sides of the LED.
-# Step 3 Insert the connecting wire to the XITANIUM converter. Plug it in.
+* Step 3 Insert the connecting wire to the XITANIUM converter. Plug it in.
 ** [[Image:SimpleProtocolResult.jpg|||100px]]