McClean:Invasive growth assay

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(Overview)
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==Overview==
==Overview==
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This is a template for how to do yeast invasive growth assay.
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This is a protocal for how to do yeast invasive growth assay.
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==Protocol==
==Protocol==

Revision as of 15:44, 8 November 2013

Contents

Overview

This is a protocal for how to do yeast invasive growth assay.

Protocol

  1. Make YPD plates with 1M Sobitol.
  2. Grow strains to saturate.
  3. Do serial dilution in 96 well plate.
  4. Frog to 1M Sobitol YPD plate.
  5. Grow the plate in 30 degree for 2-3 days, then rub the plate under steam of deionized water.
  6. Proceed to take picture.


Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. If you can't get good pictures, you can grow your rubbed plate for couple days, take picture again. Or you can grow your plate for more days before you wash it.


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References

Contact

or instead, discuss this protocol.

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