McClean:Protocols: Difference between revisions
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==Media== | ==Media== | ||
*[[McClean:KS Amino Acid Supplement | KS Amino Acid Supplement]] | |||
*[[McClean:LFM_Recipe | Low Fluorescence Media]] | *[[McClean:LFM_Recipe | Low Fluorescence Media]] | ||
*[[McClean:LFA_Membranes | Low Fluorescence Agar Membranes]] | *[[McClean:LFA_Membranes | Low Fluorescence Agar Membranes]] | ||
*[[McClean:Magic Marker Medias | Magic Marker Medias]] | *[[McClean:Magic Marker Medias | Magic Marker Medias]] | ||
*[[McClean:QuickAdeYPD | Quickie YPD supplemented with Adenine]] | |||
*[[McClean:SC_Media | Synthetic Complete (SC) Yeast Media]] | |||
==Stock Solutions== | ==Stock Solutions== | ||
Revision as of 13:10, 2 November 2011
Back to McClean Lab
Basics
Yeast
- Basic Chemostat Guide
- Bayanus Transformation
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization (Colin)
- Fluorescent in situ Hybridization (Ping)
- Mating Type Testers
- Random Spore Prep
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
- Zygote Picking
Bacteria
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- KS Amino Acid Supplement
- Low Fluorescence Media
- Low Fluorescence Agar Membranes
- Magic Marker Medias
- Quickie YPD supplemented with Adenine
- Synthetic Complete (SC) Yeast Media
Stock Solutions
- α-Factor 1mg/ml Stock
- Bacterial Glycerol 65%
- Carbenicillin Stock Solution
- Concanavalin A Solution without ions
- Cycloheximide Stock Solution
- DNA Ladder Stock for PCR
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
