McClean:Protocols

Back to McClean Lab

Basics

• Joining the Lab
• Leaving the Lab
• Lab Notebooks
• Dilution of Oligos
• Ordering Media
• Protocol Template
• Lab Database

General Lab Procedures

• Dry Ice-Ethanol Bath
• Pouring Gels for Electrophoresis (Mike)

Yeast

• Basic Chemostat Guide
• Bayanus Transformation
• Cleaning Floating Pin Replicators
• Colony PCR (Yeast)
• Fluorescence in situ Hybridization (Colin)
• Fluorescent in situ Hybridization (Ping)
• Frogging Tetrads
• Genomic DNA Prep (Bust 'n' Grab Protocol)
• GEV Strain Construction
• Glycerol stocks (yeast)
• Mating Type Testers
• Phluorin Calibration
• Pinning (96 or 384 format)
• Random Spore Prep
• Sequencing Colony PCR Product
• Smartstat Startup
• Sporulation
• Tetrad Dissection
• URA Pop-out
• Yeast Mating Halo Assay
• Yeast Nomenclature
• Yeast Recombinational Cloning
• Yeast Transformation (S. cerevisiae)
• YMC Induction in Chemostat
• Zygote Picking
• Frogging a Serial Dilution
• Plasmid Loss Assay

Bacteria

• Transformation of E. Coli
• E. coli Glycerol Stocks
• E. coli Competent Cells
• Colony PCR (E. coli)

PCR

• dNTP Stocks
• Touchdown PCR
• Takara PrimeStar PCR
• Anneal and Extend

Flow Cytometry

• General Flow Cytometry Procedure
• Cycloheximide Concentration Assay
• Flourecent Protein Folding Time Assay
• Induction time course
• Phase Plane Mapping

Media

• KS Amino Acid Supplement
• Low Fluorescence Media
• Low Fluorescence Agar Membranes
• Magic Marker Medias
• Quickie YPD supplemented with Adenine
• Synthetic Complete (SC) Yeast Media
• Synthetic Complete (SC) Media w/Drugs

Stock Solutions

• Agarose for gels
• α-Factor 1mg/ml Stock
• Bacterial Glycerol 65%
• Carbenicillin Stock Solution
• Concanavalin A Solution without ions
• Cycloheximide Stock Solution
• DNA Ladder Stock for PCR
• Geneticin/G418 Stock Solution
• PBS/0.1%Tween
• Phosphate Buffered Saline (PBS, 10x)
• Seventy Percent (70%) Ethanol

Microfluidics

• Concanavalin A Solution for Microfluidics
• Constructing Flow Cells

Microscopy

• Microscope Dos and Don'ts
• Nikon TI Basic Use
• Registering objectives on the Nikon
• 96 Well Plate Assay
• NIS Elements Repair
• New Scope Settings
• Quickie ImageJ Quantification

Misc

• 96 Well Plate Print Out
• AddGene Orders
• Sequencing
• Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)
• Orders from IDT/Genewiz/Macrogen/GenScript
• Searching a Sequence for All Database Primers