McClean:Protocols: Difference between revisions
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==Yeast== | ==Yeast== | ||
*[[McClean:Drug Concentrations | Drug Concentrations]] | |||
*[[McClean:Basic_Chemostat_Guide | Basic Chemostat Guide]] | *[[McClean:Basic_Chemostat_Guide | Basic Chemostat Guide]] | ||
*[[McClean:Bayanus Transformation | Bayanus Transformation]] | *[[McClean:Bayanus Transformation | Bayanus Transformation]] | ||
| Line 28: | Line 29: | ||
*[[McClean: GEV Strain Construction | GEV Strain Construction]] | *[[McClean: GEV Strain Construction | GEV Strain Construction]] | ||
*[[McClean: Glycerol stocks (yeast) | Glycerol stocks (yeast)]] | *[[McClean: Glycerol stocks (yeast) | Glycerol stocks (yeast)]] | ||
*[[McClean:Making and Using Frozen Yeast Competant Cells|Making and Using Frozen Yeast Competant Cells]] | |||
*[[McClean:Mating_Type_Testers | Mating Type Testers]] | *[[McClean:Mating_Type_Testers | Mating Type Testers]] | ||
*[[McClean:Phluorin Calibration | Phluorin Calibration]] | *[[McClean:Phluorin Calibration | Phluorin Calibration]] | ||
| Line 44: | Line 46: | ||
*[[McClean:Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]] | *[[McClean:Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]] | ||
*[[McClean: Zygote Picking| Zygote Picking]] | *[[McClean: Zygote Picking| Zygote Picking]] | ||
*[[McClean: Working with the LoxP/Cre System in S. cerevisiae| Working with the LoxP/Cre System in S. cerevisiae]] | |||
*[[McClean:Western Blot| Western Blot]] | *[[McClean:Western Blot| Western Blot]] | ||
*[[McClean:Membrane stripping|Membrane Stripping]] | *[[McClean:Membrane stripping|Membrane Stripping]] | ||
*[[McClean:Membrane_Stripping_Mild|Membrane Stripping-Mild]] | |||
*[[McClean:Invasive growth assay|Invasive growth assay]] | |||
==Bacteria== | ==Bacteria== | ||
| Line 52: | Line 57: | ||
*[[McClean:Competent Cells| ''E. coli'' Competent Cells]] | *[[McClean:Competent Cells| ''E. coli'' Competent Cells]] | ||
*[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]] | *[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]] | ||
*[[McClean:E. coli Electroporation | E. coli Electroporation]] | |||
==PCR== | ==PCR== | ||
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*[[McClean: Flow Cells | Constructing Flow Cells]] | *[[McClean: Flow Cells | Constructing Flow Cells]] | ||
*[[McClean: FCS2 for Cycling | FCS2 chamber for Metabolic Cycling]] | *[[McClean: FCS2 for Cycling | FCS2 chamber for Metabolic Cycling]] | ||
*[[User:Bohao Liu/Notebook/Research| Bandwidth Experiments]] | |||
==Microscopy== | ==Microscopy== | ||
| Line 101: | Line 108: | ||
*[[McClean:Scope_Settings | New Scope Settings]] | *[[McClean:Scope_Settings | New Scope Settings]] | ||
*[[McClean: Quickie ImageJ Quantification | Quickie ImageJ Quantification]] | *[[McClean: Quickie ImageJ Quantification | Quickie ImageJ Quantification]] | ||
*[[McClean: NikonTI-Eclipse Setup and operation using Micromanager|NikonTI-Eclipse Setup and operation using Micromanager]] | |||
==Misc== | ==Misc== | ||
*[[McClean:96-well_plate | 96 Well Plate Print Out]] | *[[McClean:96-well_plate | 96 Well Plate Print Out]] | ||
*[[McClean:AddGene Orders | AddGene Orders]] | *[[McClean:AddGene Orders | AddGene Orders]] | ||
*[[McClean:Blue Light Overview | Blue Light Overview]] | |||
*[[McClean:Sequencing | Sequencing]] | *[[McClean:Sequencing | Sequencing]] | ||
*[[McClean:Bibtex4Word | Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)]] | *[[McClean:Bibtex4Word | Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)]] | ||
*[[McClean:Orders from IDT/Genewiz/Macrogen/GenScript | Orders from IDT/Genewiz/Macrogen/GenScript]] | *[[McClean:Orders from IDT/Genewiz/Macrogen/GenScript | Orders from IDT/Genewiz/Macrogen/GenScript]] | ||
*[[McClean:Searching a Sequence for All Database Primers | Searching a Sequence for All Database Primers]] | *[[McClean:Searching a Sequence for All Database Primers | Searching a Sequence for All Database Primers]] | ||
*[[McClean:Annealing Oligos | Annealing Oligos]] | |||
*[[McClean:Oligonucleotide phosphorylation, Annealing and Ligation | Oligonucleotide phosphorylation, Annealing and Ligation]] | |||
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Revision as of 14:33, 18 November 2014
Back to McClean Lab
Basics
- Joining the Lab
- Leaving the Lab
- Lab Notebooks
- Dilution of Oligos
- Ordering Media
- Protocol Template
- Lab Database
General Lab Procedures
Yeast
- Drug Concentrations
- Basic Chemostat Guide
- Bayanus Transformation
- Cleaning Floating Pin Replicators
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization (Colin)
- Fluorescent in situ Hybridization (Ping)
- Fixation of Yeast (Bisaria Protocol)
- Fixation of Yeast (P. Xu Protocol)
- Frogging a Serial Dilution
- Frogging Tetrads
- Genomic DNA Prep (Bust 'n' Grab Protocol)
- GEV Strain Construction
- Glycerol stocks (yeast)
- Making and Using Frozen Yeast Competant Cells
- Mating Type Testers
- Phluorin Calibration
- Pinning (96 or 384 format)
- Plasmid Loss Assay
- Random Spore Prep
- Sequencing Colony PCR Product
- Smartstat Startup
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Mating Halo Assay
- Yeast Nomenclature
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
- Zygote Picking
- Working with the LoxP/Cre System in S. cerevisiae
- Western Blot
- Membrane Stripping
- Membrane Stripping-Mild
- Invasive growth assay
Bacteria
- Transformation of E. Coli
- E. coli Glycerol Stocks
- E. coli Competent Cells
- Colony PCR (E. coli)
- E. coli Electroporation
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- KS Amino Acid Supplement
- Low Fluorescence Media
- Low Fluorescence Agar Membranes
- Magic Marker Medias
- Quickie YPD supplemented with Adenine
- Synthetic Complete (SC) Yeast Media
- Synthetic Complete (SC) Media w/Drugs
Stock Solutions
- Agarose for gels
- α-Factor 1mg/ml Stock
- Bacterial Glycerol 65%
- Carbenicillin Stock Solution
- Concanavalin A Solution without ions
- Cycloheximide Stock Solution
- DNA Ladder Stock for PCR
- Geneticin/G418 Stock Solution
- PBS/0.1%Tween
- Phosphate Buffered Saline (PBS, 10x)
- Seventy Percent (70%) Ethanol
Microfluidics
- Concanavalin A Solution for Microfluidics
- Constructing Flow Cells
- FCS2 chamber for Metabolic Cycling
- Bandwidth Experiments
Microscopy
- Microscope Dos and Don'ts
- Nikon TI Basic Use
- Registering objectives on the Nikon
- 96 Well Plate Assay
- NIS Elements Repair
- New Scope Settings
- Quickie ImageJ Quantification
- NikonTI-Eclipse Setup and operation using Micromanager
Misc
- 96 Well Plate Print Out
- AddGene Orders
- Blue Light Overview
- Sequencing
- Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)
- Orders from IDT/Genewiz/Macrogen/GenScript
- Searching a Sequence for All Database Primers
- Annealing Oligos
- Oligonucleotide phosphorylation, Annealing and Ligation
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