McClean:Protocols: Difference between revisions

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==Basics==
==Basics==
*[[McClean: Lab Expectations | Lab Expectations]]
*[[McClean: Lab Rules | Lab Rules]]
*[[McClean: Lab Jobs| Lab Jobs]]
*[[McClean: Joining The Lab |Joining the Lab]]
*[[McClean: Leaving The Lab | Leaving the Lab]]
*[[McClean: Lab Notebooks | Lab Notebooks]]
*[[McClean: Lab Notebooks | Lab Notebooks]]
*[[McClean: Dilution_of_Oligos | Dilution of Oligos]]
*[[McClean: Dilution_of_Oligos | Dilution of Oligos]]
*[[McClean: Ordering_Media | Ordering Media]]
*[[McClean: Order Supplies | Ordering Supplies]]
*[[McClean: Protocol Template| Protocol Template]]
*[[McClean: Lab Database | Lab Database]]
*[[McClean: Lab Organization Notes | Lab Organization Notes]]


==Yeast==
==Introductory Exercises==
*[[McClean:Random_Spore_Prep | Random Spore Prep]]
*[[McClean: Intro_to_yeast | Introduction to Yeast]]
 
==Health and Safety==
*[[McClean: Safety101 | Safety 101]]
*[[McClean: OkToTrash | Ok to Trash Stickers]]


*[[McClean:Yeast Transformation | Yeast Transformation]]
==General Lab Procedures==
*[[McClean: Washing Glassware | Washing Glassware]]
*[[McClean: Dry Ice-Ethanol Bath | Dry Ice-Ethanol Bath]]
*[[McClean: Pouring Gels for Electrophoresis | Pouring Gels for Electrophoresis (Mike)]]
*[[McClean: Nanodrop2000 | Using the Nanodrop2000]]
*[[McClean:Qubit | Quantifying DNA with the Qubit Fluorometer]]
*[[McClean: OrderingLiquidN2 | Ordering Liquid N2 for Common Lab]]


==Yeast==
*[[McClean:Drug Concentrations | Drug Concentrations]]
*[[McClean:Basic_Chemostat_Guide | Basic Chemostat Guide]]
*[[McClean:Bayanus Transformation | Bayanus Transformation]]
*[[McClean: Cleaning Floating Pin Replicators | Cleaning Floating Pin Replicators]]
*[[McClean:FISH | Fluorescence in situ Hybridization  (Colin)]]
*[[McClean: FISH (Ping) | Fluorescent in situ Hybridization (Ping)]]
*[[McClean: FISH (Gasch) | Fluorescent in situ Hybridization (Gasch Lab Collaboration)]]
*[[McClean: Fixation of Yeast (Bisaria Protocol) | Fixation of Yeast (Bisaria Protocol)]]
*[[McClean: Fixation of Yeast (McClean Protocol) | Fixation of Yeast (McClean Protocol)]]
*[[McClean: Fixation of Yeast (P. Xu Protocol) | Fixation of Yeast (P. Xu Protocol)]]
*[[McClean: Frogging a Serial Dilution| Frogging a Serial Dilution]]
*[[McClean: Frogging_Tetrads | Frogging Tetrads]]
*[[McClean: Genomic DNA Prep (Bust 'n' Grab Protocol)| Genomic DNA Prep (Bust 'n' Grab Protocol)]]
*[[McClean: GEV Strain Construction | GEV Strain Construction]]
*[[McClean: Glycerol stocks (yeast) | Glycerol stocks (yeast)]]
*[[McClean: Hemacytometer protocol for yeast | Hemacytometer protocol for yeast]]
*[[McClean:Making and Using Frozen Yeast Competant Cells|Making and Using Frozen Yeast Competant Cells]]
*[[McClean:Mating_Type_Testers | Mating Type Testers]]
*[[McClean:Phluorin Calibration | Phluorin Calibration]]
*[[McClean: Pinning (96 or 384 format) | Pinning (96 or 384 format)]]
*[[McClean: Plasmid Loss Assay| Plasmid Loss Assay]]
*[[McClean:Random_Spore_Prep |Random Spore Prep]]
*[[McClean: Sequencing Colony PCR Product | Sanger Sequencing]]
*[[McClean:Smartstat Startup | Smartstat Startup]]
*[[McClean: Sporulation | Sporulation]]
*[[McClean: Tetrad Dissection | Tetrad Dissection]]
*[[McClean:URA Pop-out | URA Pop-out]]
*[[McClean:URA Pop-out | URA Pop-out]]
 
*[[McClean: Yeast Dubious ORFs | Yeast Dubious ORFs]]
*[[McClean:Yeast Recombinational Cloning | Yeast Recombinational Cloning]]
*[[McClean: Yeast Mating Halo Assay | Yeast Mating Halo Assay]]
 
*[[McClean: Yeast Nomenclature | Yeast Nomenclature]]
*[[McClean:FISH | Fluorescence in situ Hybridization]]
*[[McClean: Yeast Recombinational Cloning | Yeast Recombinational Cloning]]
*[[McClean:Yeast Transformation | Yeast Transformation (S. cerevisiae)]]
*[[McClean:Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]]
*[[McClean: Zygote Picking| Zygote Picking]]
*[[McClean: Working with the LoxP/Cre System in S. cerevisiae| Working with the LoxP/Cre System in S. cerevisiae]]
*[[McClean:Western Blot| Western Blot]]
*[[McClean:Membrane stripping|Membrane Stripping]]
*[[McClean:Membrane_Stripping_Mild|Membrane Stripping-Mild]]
*[[McClean:Invasive growth assay|Invasive growth assay]]


==Bacteria==
==Bacteria==
*[[McClean:Transformation of E. Coli | Transformation of ''E. Coli'']]
*[[McClean:Transformation of E. Coli | Transformation of ''E. Coli'']]
*[[McClean:E. Coli Glycerol Stocks | ''E. Coli'' Glycerol Stocks]]
*[[McClean:E. coli Glycerol Stocks | ''E. coli'' Glycerol Stocks]]
*[[McClean:Competent Cells| ''E. coli'' Competent Cells]]
*[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]]
*[[McClean:E. coli Electroporation | E. coli Electroporation]]
*[[McClean: PlasmidPrep_QiagenKit | Plasmid Prep from E. coli using a Qiagen Kit]]


==PCR==
==PCR==
*[[McClean: designing primers | Designing Primers (Yeast)]]
*[[McClean: Colony PCR (Yeast) | Colony PCR (Yeast)]]
*[[McClean:dNTPs | dNTP Stocks]]
*[[McClean:dNTPs | dNTP Stocks]]
*[[McClean:Touchdown_PCR | Touchdown PCR]]
*[[McClean:Touchdown_PCR | Touchdown PCR]]
*[[McClean: Takara PrimeStar PCR | Takara PrimeStar PCR]]
*[[McClean: Anneal and Extend | Anneal and Extend]]
*[[McClean: Designing "Yeast Toolkit" compatible primers | Designing "Yeast Toolkit" compatible primers]]
==Other DNA Manipulations==
*[[McClean: Golden Gate-Making a new part | Golden Gate-Making a new part]]
*[[McClean: Golden Gate- Making a multi-part plasmid | Golden Gate-Making a multi-part plasmid]]


==Flow Cytometry==
==Flow Cytometry==
*[[McClean:General_Flow_Cytometry_Procedure | General Flow Cytometry Procedure]]
*[[McClean:Cycloheximide_Concentration | Cycloheximide Concentration Assay]]
*[[McClean:_Fluorescent_Folding_Time_Assay | Flourecent Protein Folding Time Assay]]
*[[McClean:_Fluorescent_Folding_Time_Assay | Flourecent Protein Folding Time Assay]]
*[[McClean:General_Flow_Cytometry_Procedure | General Flow Cytometry Procedure]]
*[[McClean:Induction_Timecourse | Induction time course]]
*[[McClean:Phase_Plane_Mapping | Phase Plane Mapping]]


==Media==
==Media==
*[[McClean:KS Amino Acid Supplement | KS Amino Acid Supplement]]
*[[McClean:LB1 | LB1]]
*[[McClean:LB2 | LB2]]
*[[McClean:LFM_Recipe | Low Fluorescence Media]]
*[[McClean:LFM_Recipe | Low Fluorescence Media]]
*[[McClean:LFA_Membranes | Low Fluorescence Agar Membranes]]
*[[McClean:Magic Marker Medias | Magic Marker Medias]]
*[[McClean:QuickAdeYPD | Quickie YPD supplemented with Adenine]]
*[[McClean:QuickAdeYPD | Quickie YPD supplemented with Adenine]]
*[[McClean:LFA_Membranes | Low Fluorescence Agar Membranes]]
*[[McClean:SC_Media | Synthetic Complete (SC) Yeast Media]]
*[[McClean:SC_MediawDrugs | Synthetic Complete (SC) Media w/Drugs]]
*[[McClean:SC_Canavanine | Synthetic Complete (SC) Media w/Canavanine]]
*[[McClean:YNB1 | YNB1]]
*[[McClean:YNB2 | YNB2]]
*[[McClean:YNB3 | YNB3]]
*[[McClean:YNB4 | YNB4]]
*[[McClean:YPD2 | YPD2]]
*[[McClean:YPD4 | YPD4]]
*[[McClean:Chloramphenicol media | Chloramphenicol media]]
*[[McClean:Kanamycin media | Kanamycin media]]
*[[McClean:5FOA | 5FOA]]


==Stock Solutions==
==Stock Solutions==
*[[McClean: Agarose for gels | Agarose for gels]]
*[[McClean: Alpha Factor Stock | α-Factor 1mg/ml Stock]]
*[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]]
*[[McClean: Carbenicillin Stock Solution| Carbenicillin Stock Solution]]
*[[McClean: Carbenicillin Stock Solution| Carbenicillin Stock Solution]]
*[[McClean: FISH ConA| Concanavalin A Solution without ions]]
*[[McClean: Cycloheximide Stock Solution| Cycloheximide Stock Solution]]
*[[McClean: Ladder_Stock_for_PCR | DNA Ladder Stock for PCR]]
*[[McClean: EDTA (0.5M) | EDTA (0.5M)]]
*[[McClean: Geneticin/G418 Stock Solution | Geneticin/G418 Stock Solution]]
*[[McClean: Glucose Solution | Glucose Solution for Media]]
*[[McClean: PBS/0.1%Tween| PBS/0.1%Tween]]
*[[McClean: Phosphate Buffered Saline| Phosphate Buffered Saline (PBS, 10x)]]
*[[McClean: Potassium Phosphate | Potassium Phosphate Buffer]]
*[[McClean: SeventyPercent_EtOH | Seventy Percent (70%) Ethanol]]
*[[McClean: TAE_50X | TAE Buffer (50X)]]
*[[McClean: TE_10x | TE Buffer (10X)]]
*[[McClean: Tris_Buffer | Tris Buffer]]
*[[McClean: Yeast glycerol | Yeast Glycerol 30%]]
*[[McClean:Chloramphenicol | Chloramphenicol]]
*[[McClean:Kanamycin | Kanamycin]]


*[[McClean: Ladder_Stock_for_PCR | DNA Ladder Stock for PCR]]
==Microfluidics==
*[[McClean: ConA | Concanavalin A Solution for Microfluidics]]
*[[McClean: Flow Cells | Constructing PDMS Flow Cells]]
*[[McClean: FCS2 for Cycling | FCS2 chamber for Metabolic Cycling]]
*[[User:Bohao Liu/Notebook/Research| Bandwidth Experiments]]
*[[McClean: BioptechsTempExp | Bioptechs Temperature Experiments]]


==Microscopy==
==Microscopy==
*[[McClean: Intro to the microscope| Introduction to the microscope]]
*[[McClean: Microscope Dos and Don'ts| Microscope Dos and Don'ts]]
*[[McClean: Nikon_TI_Basic_Use | Nikon TI Basic Use]]
*[[McClean: Nikon_TI_Basic_Use | Nikon TI Basic Use]]
*[[McClean: Registering objectives on the Nikon | Registering objectives on the Nikon]]
*[[McClean: 96_Well_Plate_Assay | 96 Well Plate Assay]]
*[[McClean: 96_Well_Plate_Assay | 96 Well Plate Assay]]
*[[McClean: NIS Elements Repair | NIS Elements Repair]]
*[[McClean:Scope_Settings | New Scope Settings]]
*[[McClean:Scope_Settings | New Scope Settings]]
*[[McClean: Quickie ImageJ Quantification | Quickie ImageJ Quantification]]
*[[McClean: NikonTI-Eclipse Setup and operation using Micromanager|NikonTI-Eclipse Setup and operation using Micromanager]]
*[[McClean: CleaningTheCamera| Cleaning the Camera's Cover]]
==Equipment Maintenance==
*[[McClean: RollerDrum_BrushReplacement | Roller Drum Brush Replacement]]
==Misc==
*[[McClean:96-well_plate | 96 Well Plate Print Out]]
*[[McClean:AddGene Orders | AddGene Orders]]
*[[McClean:Blue Light Overview | Blue Light Overview]]
*[[McClean:Sequencing | Sequencing]]
*[[McClean:Bibtex4Word | Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)]]
*[[McClean:Orders from IDT/Genewiz/Macrogen/GenScript | Orders from IDT/Genewiz/Macrogen/GenScript]]
*[[McClean:Searching a Sequence for All Database Primers | Searching a Sequence for All Database Primers]]
*[[McClean:Annealing Oligos | Annealing Oligos]]
*[[McClean:Oligonucleotide phosphorylation, Annealing and Ligation | Oligonucleotide phosphorylation, Annealing and Ligation]]
*[[McClean:BlueLightSetup | How to Connect Blue LED to a Power Source, Parts and Instructions]]
*[[McClean:InstallKappa |Compiling Kappa in a Linux environment]]
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Revision as of 13:41, 22 February 2017

Back to McClean Lab

Basics

Introductory Exercises

Health and Safety

General Lab Procedures

Yeast

Bacteria

PCR

Other DNA Manipulations

Flow Cytometry

Media

Stock Solutions

Microfluidics

Microscopy

Equipment Maintenance

Misc

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