McClean:Protocols: Difference between revisions

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==Basics==
==Basics==
*[[McClean: Lab Expectations | Lab Expectations]]
*[[McClean: Lab Rules | Lab Rules]]
*[[McClean: Lab Jobs| Lab Jobs]]
*[[McClean: Joining The Lab |Joining the Lab]]
*[[McClean: Joining The Lab |Joining the Lab]]
*[[McClean: Leaving The Lab | Leaving the Lab]]
*[[McClean: Leaving The Lab | Leaving the Lab]]
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*[[McClean: Dilution_of_Oligos | Dilution of Oligos]]
*[[McClean: Dilution_of_Oligos | Dilution of Oligos]]
*[[McClean: Ordering_Media | Ordering Media]]
*[[McClean: Ordering_Media | Ordering Media]]
*[[McClean: Order Supplies | Ordering Supplies]]
*[[McClean: Protocol Template| Protocol Template]]
*[[McClean: Protocol Template| Protocol Template]]
*[[McClean: Lab Database | Lab Database]]
*[[McClean: Lab Database | Lab Database]]
*[[McClean: Lab Organization Notes | Lab Organization Notes]]
==Introductory Exercises==
*[[McClean: Intro_to_yeast | Introduction to Yeast]]
*[[McClean: Tester Protocol | Tester Protocol]]
==Health and Safety==
*[[McClean: Safety101 | Safety 101]]
*[[McClean: SharpsDisposal | Sharps Disposal]]
*[[McClean: OkToTrash | Ok to Trash Stickers]]
==General Lab Procedures==
*[[McClean: Washing Glassware | Washing Glassware]]
*[[McClean: Dry Ice-Ethanol Bath | Dry Ice-Ethanol Bath]]
*[[McClean: Pouring Gels for Electrophoresis | Pouring Gels for Electrophoresis (Mike)]]
*[[McClean: Nanodrop2000 | Using the Nanodrop2000]]
*[[McClean:Qubit | Quantifying DNA with the Qubit Fluorometer]]
*[[McClean: OrderingLiquidN2 | Ordering Liquid N2 for Common Lab]]
*[[McClean: Using the Klett | Klett protocols]]


==Yeast==
==Yeast==
*[[McClean:Drug Concentrations | Drug Concentrations]]
*[[McClean:Basic_Chemostat_Guide | Basic Chemostat Guide]]
*[[McClean:Basic_Chemostat_Guide | Basic Chemostat Guide]]
*[[McClean:Bayanus Transformation | Bayanus Transformation]]
*[[McClean:Bayanus Transformation | Bayanus Transformation]]
*[[McClean:Cleaning Floating Pin Replicators | Cleaning Floating Pin Replicators]]
*[[McClean: Cleaning Floating Pin Replicators | Cleaning Floating Pin Replicators]]
*[[McClean:Colony PCR (Yeast) | Colony PCR (Yeast)]]
*[[McClean:FISH | Fluorescence in situ Hybridization  (Colin)]]
*[[McClean:FISH | Fluorescence in situ Hybridization  (Colin)]]
*[[McClean:FISH (Ping) | Fluorescent in situ Hybridization (Ping)]]
*[[McClean: FISH (Ping) | Fluorescent in situ Hybridization (Ping)]]
*[[McClean:Frogging_Tetrads | Frogging Tetrads]]
*[[McClean: FISH (Gasch) | Fluorescent in situ Hybridization (Gasch Lab Collaboration)]]
*[[McClean: Fixation of Yeast (Bisaria Protocol) | Fixation of Yeast (Bisaria Protocol)]]
*[[McClean: Fixation of Yeast (McClean Protocol) | Fixation of Yeast (McClean Protocol)]]
*[[McClean: Fixation of Yeast (P. Xu Protocol) | Fixation of Yeast (P. Xu Protocol)]]
*[[McClean: Frogging a Serial Dilution| Frogging a Serial Dilution]]
*[[McClean: Frogging_Tetrads | Frogging Tetrads]]
*[[McClean: Genomic DNA Prep (Bust 'n' Grab Protocol)| Genomic DNA Prep (Bust 'n' Grab Protocol)]]
*[[McClean:Genomic_DNA_prep_(LiAc_SDS_method)| Genomic DNA Prep (LiAc SDS method)]]
*[[McClean: GEV Strain Construction | GEV Strain Construction]]
*[[McClean: Glycerol stocks (yeast) | Glycerol stocks (yeast)]]
*[[McClean: Hemacytometer protocol for yeast | Hemacytometer protocol for yeast]]
*[[McClean:Making and Using Frozen Yeast Competant Cells|Making and Using Frozen Yeast Competant Cells]]
*[[McClean:Mating_Type_Testers | Mating Type Testers]]
*[[McClean:Mating_Type_Testers | Mating Type Testers]]
*[[McClean:Pinning (96 or 384 format) | Pinning (96 or 384 format)]]
*[[McClean:Phluorin Calibration | Phluorin Calibration]]
*[[McClean: Pinning (96 or 384 format) | Pinning (96 or 384 format)]]
*[[McClean: Plasmid Loss Assay| Plasmid Loss Assay]]
*[[McClean:Random_Spore_Prep |Random Spore Prep]]
*[[McClean:Random_Spore_Prep |Random Spore Prep]]
*[[McClean:Sporulation | Sporulation]]
*[[McClean: Sequencing Colony PCR Product | Sanger Sequencing]]
*[[McClean:Tetrad Dissection | Tetrad Dissection]]
*[[McClean:Smartstat Startup | Smartstat Startup]]
*[[McClean: Sonication_Yeast | Sonication of Yeast]]
*[[McClean: Sporulation | Sporulation]]
*[[McClean: Tetrad Dissection | Tetrad Dissection]]
*[[McClean:URA Pop-out | URA Pop-out]]
*[[McClean:URA Pop-out | URA Pop-out]]
*[[McClean:Yeast Mating Halo Assay | Yeast Mating Halo Assay]]
*[[McClean: Yeast Dubious ORFs | Yeast Dubious ORFs]]
*[[McClean:Yeast Nomenclature | Yeast Nomenclature]]
*[[McClean: Yeast Mating Halo Assay | Yeast Mating Halo Assay]]
*[[McClean:Yeast Recombinational Cloning | Yeast Recombinational Cloning]]
*[[McClean: Yeast Nomenclature | Yeast Nomenclature]]
*[[McClean: Yeast Recombinational Cloning | Yeast Recombinational Cloning]]
*[[McClean:Yeast Transformation | Yeast Transformation (S. cerevisiae)]]
*[[McClean:Yeast Transformation | Yeast Transformation (S. cerevisiae)]]
*[[McClean:Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]]
*[[McClean:Chemostat_Metabolic_Cycle | YMC Induction in Chemostat]]
*[[McClean:Zygote Picking| Zygote Picking]]
*[[McClean: Zygote Picking| Zygote Picking]]
*[[McClean: Working with the LoxP/Cre System in S. cerevisiae| Working with the LoxP/Cre System in S. cerevisiae]]
*[[McClean:EMSA Protocol| EMSA Protocol]]
*[[McClean:Western Blot| Western Blot]]
*[[McClean:Membrane stripping|Membrane Stripping]]
*[[McClean:Membrane_Stripping_Mild|Membrane Stripping-Mild]]
*[[McClean:Invasive growth assay|Invasive growth assay]]
*[[McClean:Yeast_viability_with_methylene_blue|Methylene blue viability assay]]


==Bacteria==
==Bacteria==
*[[McClean:Transformation of E. Coli | Transformation of ''E. Coli'']]
*[[McClean:Transformation of E. Coli | Transformation of ''E. Coli'']]
*[[McClean:E. coli Glycerol Stocks | ''E. coli'' Glycerol Stocks]]
*[[McClean:E. coli Glycerol Stocks | ''E. coli'' Glycerol Stocks]]
*[[McClean:Competent Cells| ''DH5alpha'' Competent Cells with Mix & Go kit (Renee)]]
*[[McClean:Competent Cells| ''E. coli'' Competent Cells]]
*[[McClean:Competent Cells| ''E. coli'' Competent Cells]]
*[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]]
*[[McClean:Colony PCR(E. coli) | Colony PCR (''E. coli'')]]
*[[McClean:E. coli Electroporation | E. coli Electroporation]]
*[[McClean: PlasmidPrep_QiagenKit | Plasmid Prep from E. coli using a Qiagen Kit]]


==PCR==
==PCR==
*[[McClean: designing primers | Designing Primers (Yeast)]]
*[[McClean: Colony PCR (Yeast) | Colony PCR (Yeast)]]
*[[McClean:dNTPs | dNTP Stocks]]
*[[McClean:dNTPs | dNTP Stocks]]
*[[McClean:Touchdown_PCR | Touchdown PCR]]
*[[McClean:Touchdown_PCR | Touchdown PCR]]
*[[McClean: Takara PrimeStar PCR | Takara PrimeStar PCR]]
*[[McClean: Anneal and Extend | Anneal and Extend]]
*[[McClean: Designing "Yeast Toolkit" compatible primers | Designing "Yeast Toolkit" compatible primers]]
*[[McClean: Q5 Mutagenesis Kit from NEB | Q5 Mutagenesis Kit from NEB]]
*[[McClean:Colony_PCR_(Quick_Load) | Colony PCR (Quick Load)]]
==Modular Cloning-Yeast Toolkit assembly==
*[[McClean: Designing "Yeast Toolkit" compatible primers | Designing "Yeast Toolkit" compatible primers]]
*[[McClean: Golden Gate-Making a new part | Golden Gate-Making a new part]]
*[[McClean: Golden Gate- Making a multi-part plasmid | Golden Gate-Making a multi-part plasmid]]


==Flow Cytometry==
==Flow Cytometry==
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==Media==
==Media==
*[[McClean:Chloramphenicol media | Chloramphenicol media]]
*[[McClean:Kanamycin media | Kanamycin media]]
*[[McClean:KS Amino Acid Supplement | KS Amino Acid Supplement]]
*[[McClean:KS Amino Acid Supplement | KS Amino Acid Supplement]]
*[[McClean:LB1 | LB1]]
*[[McClean:LB2 | LB2]]
*[[McClean:LFM_Recipe | Low Fluorescence Media]]
*[[McClean:LFM_Recipe | Low Fluorescence Media]]
*[[McClean:LFA_Membranes | Low Fluorescence Agar Membranes]]
*[[McClean:LFA_Membranes | Low Fluorescence Agar Membranes]]
*[[McClean:Magic Marker Medias | Magic Marker Medias]]
*[[McClean:Magic Marker Medias | Magic Marker Medias]]
*[[McClean:QuickAdeYPD | Quickie YPD supplemented with Adenine]]
*[[McClean:QuickAdeYPD | Quickie YPD supplemented with Adenine]]
*[[McClean:YPG | YPG]]
*[[McClean:SC_Media | Synthetic Complete (SC) Yeast Media]]
*[[McClean:SC_Media | Synthetic Complete (SC) Yeast Media]]
*[[McClean:SC_MediawDrugs | Synthetic Complete (SC) Media w/Drugs]]
*[[McClean:SC_MediawDrugs | Synthetic Complete (SC) Media w/Drugs]]
*[[McClean:SC_Canavanine | Synthetic Complete (SC) Media w/Canavanine]]
*[[McClean:YNB1 | YNB1]]
*[[McClean:YNB2 | YNB2]]
*[[McClean:YNB3 | YNB3]]
*[[McClean:YNB4 | YNB4]]
*[[McClean:YPD2 | YPD2]]
*[[McClean:YPD4 | YPD4]]


==Stock Solutions==
==Stock Solutions==
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*[[McClean: Alpha Factor Stock | α-Factor 1mg/ml Stock]]
*[[McClean: Alpha Factor Stock | α-Factor 1mg/ml Stock]]
*[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]]
*[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]]
*[[McClean: Carbenicillin Stock Solution| Carbenicillin Stock Solution]]
*[[McClean: FISH ConA| Concanavalin A Solution without ions]]
*[[McClean: FISH ConA| Concanavalin A Solution without ions]]
*[[McClean: Cycloheximide Stock Solution| Cycloheximide Stock Solution]]
*[[McClean: Ladder_Stock_for_PCR | DNA Ladder Stock for PCR]]
*[[McClean: Ladder_Stock_for_PCR | DNA Ladder Stock for PCR]]
*[[McClean: Geneticin/G418 Stock Solution | Geneticin/G418 Stock Solution]]
*[[McClean: EDTA (0.5M) | EDTA (0.5M)]]
*[[McClean: Glucose Solution | Glucose Solution for Media]]
*[[McClean: PBS/0.1%Tween| PBS/0.1%Tween]]
*[[McClean: PBS/0.1%Tween| PBS/0.1%Tween]]
*[[McClean: Phosphate Buffered Saline| Phosphate Buffered Saline (PBS, 10x)]]
*[[McClean: Phosphate Buffered Saline| Phosphate Buffered Saline (PBS, 10x)]]
*[[McClean: Potassium Phosphate | Potassium Phosphate Buffer]]
*[[McClean: Plasmid Prep Buffers | Generic recipes for Qiagen buffers]]
*[[McClean: SeventyPercent_EtOH | Seventy Percent (70%) Ethanol]]
*[[McClean: SeventyPercent_EtOH | Seventy Percent (70%) Ethanol]]
*[[McClean: TAE_50X | TAE Buffer (50X)]]
*[[McClean: TE_10x | TE Buffer (10X)]]
*[[McClean: Tris_Buffer | Tris Buffer]]
*[[McClean: Yeast glycerol | Yeast Glycerol 30%]]
==Drugs==
*[[McClean:Drug Concentrations | Drug Concentrations]]
===E.Coli===
*[[McClean:Carbenicillin Stock Solution | Carbenicillin Stock Solution]]
*[[McClean:Chloramphenicol | Chloramphenicol]]
*[[McClean:Gentamicin | Gentamicin]]
*[[McClean:Kanamycin | Kanamycin]]
*[[McClean:Spectinomycin | Spectinomycin]]
===S.cerevisiae===
*[[McClean:5FOA | 5FOA]]
*[[McClean:ClonNat | ClonNat]]
*[[McClean: Cycloheximide Stock Solution| Cycloheximide Stock Solution]]
*[[McClean: Geneticin/G418 Stock Solution | Geneticin/G418 Stock Solution]]
*[[McClean:Hygromycin B | Hygromycin B]]


==Microfluidics==
==Microfluidics==
*[[McClean: ConA | Concanavalin A Solution for Microfluidics]]
*[[McClean: ConA | Concanavalin A Solution for Microfluidics]]
*[[McClean: Flow Cells | Constructing PDMS Flow Cells]]
*[[McClean: FCS2 for Cycling | FCS2 chamber for Metabolic Cycling]]
*[[User:Bohao Liu/Notebook/Research| Bandwidth Experiments]]
*[[McClean: BioptechsTempExp | Bioptechs Temperature Experiments]]
==Microscopy==
==Microscopy==
*[[McClean: Intro to the microscope| Introduction to the microscope]]
*[[McClean: Microscope Dos and Don'ts| Microscope Dos and Don'ts]]
*[[McClean: Microscope Dos and Don'ts| Microscope Dos and Don'ts]]
*[[McClean: Nikon_TI_Basic_Use | Nikon TI Basic Use]]
*[[McClean: Nikon_TI_Basic_Use | Nikon TI Basic Use]]
*[[McClean: Registering objectives on the Nikon | Registering objectives on the Nikon]]
*[[McClean: 96_Well_Plate_Assay | 96 Well Plate Assay]]
*[[McClean: 96_Well_Plate_Assay | 96 Well Plate Assay]]
*[[McClean: NIS Elements Repair | NIS Elements Repair]]
*[[McClean: NIS Elements Repair | NIS Elements Repair]]
*[[McClean:Scope_Settings | New Scope Settings]]
*[[McClean:Scope_Settings | New Scope Settings]]
*[[McClean: Quickie ImageJ Quantification | Quickie ImageJ Quantification]]
*[[McClean: Quickie ImageJ Quantification | Quickie ImageJ Quantification]]
*[[McClean: NikonTI-Eclipse Setup and operation using Micromanager|NikonTI-Eclipse Setup and operation using Micromanager]]
*[[McClean: CleaningTheCamera| Cleaning the Camera's Cover]]
==Equipment Maintenance==
*[[McClean: Laboratory_Cabinet_Maint | Laboratory Cabinet Maintenance]]
*[[McClean: RollerDrum_BrushReplacement | Roller Drum Brush Replacement]]
*[[McClean: PlasmaPreen_ORingReplacement | Plasma Preen O-Ring Replacement]]
*[[McClean: LowDiskSpace | Microscope #1 computer -- Low Disk Space Warning]]


==Misc==
==Misc==
*[[McClean:96-well_plate | 96 Well Plate Print Out]]
*[[McClean:96-well_plate | 96 Well Plate Print Out]]
*[[McClean:AddGene Orders | AddGene Orders]]
*[[McClean:AddGene Orders | AddGene Orders]]
*[[McClean:Blue Light Overview | Blue Light Overview]]
*[[McClean:Sequencing | Sequencing]]
*[[McClean:Sequencing | Sequencing]]
*[[McClean:Bibtex4Word | Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)]]
*[[McClean:Bibtex4Word | Bibtex4Word (Add-in for word that allows you to use your existing BibTeX database)]]
*[[McClean:Orders from IDT/Genewiz/Macrogen/GenScript | Orders from IDT/Genewiz/Macrogen/GenScript]]
*[[McClean:Searching a Sequence for All Database Primers | Searching a Sequence for All Database Primers]]
*[[McClean:Annealing Oligos | Annealing Oligos]]
*[[McClean:Oligonucleotide phosphorylation, Annealing and Ligation | Oligonucleotide phosphorylation, Annealing and Ligation]]
*[[McClean:BlueLightSetup | How to Connect Blue LED to a Power Source, Parts and Instructions]]
*[[McClean:InstallKappa |Compiling Kappa in a Linux environment]]
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Latest revision as of 12:48, 5 February 2024

Back to McClean Lab

Basics

Introductory Exercises

Health and Safety

General Lab Procedures

Yeast

Bacteria

PCR

Modular Cloning-Yeast Toolkit assembly

Flow Cytometry

Media

Stock Solutions

Drugs

E.Coli

S.cerevisiae

Microfluidics

Microscopy

Equipment Maintenance

Misc

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