McClean:Protocols

Back to McClean Lab

Basics

• Joining the Lab
• Leaving the Lab
• Lab Notebooks
• Dilution of Oligos
• Ordering Media
• Protocol Template

Yeast

• Basic Chemostat Guide
• Bayanus Transformation
• Colony PCR (Yeast)
• Fluorescence in situ Hybridization
• Mating Type Testers
• Random Spore Prep
• Sporulation
• Tetrad Dissection
• URA Pop-out
• Yeast Recombinational Cloning
• Yeast Transformation (S. cerevisiae)
• YMC Induction in Chemostat

Bacteria

• Transformation of E. Coli
• E. Coli Glycerol Stocks

PCR

• dNTP Stocks
• Touchdown PCR

Flow Cytometry

• General Flow Cytometry Procedure
• Cycloheximide Concentration Assay
• Flourecent Protein Folding Time Assay
• Induction time course
• Phase Plane Mapping

Media

• Low Fluorescence Media
• Quickie YPD supplemented with Adenine
• Low Fluorescence Agar Membranes
• KS Amino Acid Supplement

Stock Solutions

• Carbenicillin Stock Solution

• DNA Ladder Stock for PCR
• Bacterial Glycerol 65%

Microfluidics

• Concanavalin A Solution for Microfluidics

Microscopy

• Nikon TI Basic Use
• 96 Well Plate Assay
• New Scope Settings

Misc

• 96 Well Plate Print Out
• AddGene Orders
• Sequencing