McClean:SC Media - Revision history

Megan N McClean: /* Stock Solutions */

Megan N McClean — Thu, 03 Nov 2011 13:39:07 GMT

Stock Solutions

←Older revision		Revision as of 13:39, 3 November 2011
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	For 1 liter of 20% glucose:		For 1 liter of 20% glucose:
-	*Add 200g Dextrose (Sigma D9434) to 400ml of deionized water ~~with~~ with constant stirring. Add the water to the beaker first! Heat may help the sugar to dissolve	+	*Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with constant stirring. Add the water to the beaker first then sugar! Heat may help the sugar to dissolve.
	*Once the sugar has dissolved, bring the entire solution up to 1 liter. Divide into smaller bottles (usually you will use 50mls of 20% glucose to make 1L of media, so we usually aliquot about 100mls into small bottles). Autoclave for 30 minutes on the liquid cycle.		*Once the sugar has dissolved, bring the entire solution up to 1 liter. Divide into smaller bottles (usually you will use 50mls of 20% glucose to make 1L of media, so we usually aliquot about 100mls into small bottles). Autoclave for 30 minutes on the liquid cycle.
		+	*We store stock solutions of 10x glucose by the microwave in Room 115.

	''10x Amino acid mix''		''10x Amino acid mix''

Megan N McClean: /* Notes */

Megan N McClean — Wed, 02 Nov 2011 21:32:36 GMT

Notes

←Older revision		Revision as of 21:32, 2 November 2011
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	==Notes==		==Notes==
	<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->		<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->
-	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!	+	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
-	*[[User:Megan N McClean\|Megan N McClean]] 17:32, 2 November 2011 (EDT) Once the bacto-agar and nitroge base have been autoclaved, they can be allowed to solidify. ~~In this state they can be stored for several months. To make plates, microwave the solidified YNB agar, allow to cool to ~55°C in a water bath, add the glucose and amino acids, and pour plates.~~	+
-		+
	Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.		Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
		+
		+	*[[User:Megan N McClean\|Megan N McClean]] 17:32, 2 November 2011 (EDT) Once the bacto-agar and nitroge base have been autoclaved, they can be allowed to solidify. In this state they can be stored for several months. To make plates, microwave the solidified YNB agar, allow to cool to ~55°C in a water bath, add the glucose and amino acids, and pour plates.

	==References==		==References==

Megan N McClean: /* Notes */

Megan N McClean — Wed, 02 Nov 2011 21:32:15 GMT

Notes

←Older revision		Revision as of 21:32, 2 November 2011
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	<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->		<!-- Please paste this section "as is" into your protocol, and add notes to it if you have some!-->
	Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!		Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
-	~~#List troubleshooting tips here.~~	+	*[[User:Megan N McClean\|Megan N McClean]] 17:32, 2 November 2011 (EDT) Once the bacto-agar and nitroge base have been autoclaved, they can be allowed to solidify. In this state they can be stored for several months. To make plates, microwave the solidified YNB agar, allow to cool to ~55°C in a water bath, add the glucose and amino acids, and pour plates.
-	~~#You can also link to FAQs/tips provided by other sources such as~~ the ~~manufacturer or other websites.~~	+
-	~~#Anecdotal observations that might~~ be ~~of use~~ to ~~others~~ can ~~also~~ be ~~posted here~~.	+

	Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.		Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.

Megan N McClean: /* Protocol */

Megan N McClean — Wed, 02 Nov 2011 21:30:23 GMT

Protocol

←Older revision		Revision as of 21:30, 2 November 2011
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	==Protocol==		==Protocol==
-	For 1 liter of media mix together:	+	For 1 liter of solid media mix together:
	*6.7g yeast nitrogen base		*6.7g yeast nitrogen base
	*20g bacto-agar		*20g bacto-agar
	*deionized water to 900ml		*deionized water to 900ml
-	Autoclave for 30 minutes on the liquid cycle. Once cooled ~~(but see Notes)~~ to about 55°C add 50ml of the 10x glucose solution and 50ml of the 10x amino acid mix. Pour plates.	+	Autoclave for 30 minutes on the liquid cycle. Once cooled to about 55°C add 50ml of the 10x glucose solution and 50ml of the 10x amino acid mix. Pour plates.
-		+


		+	For 1 liter of liquid media mix together:
		+	*6.7g yeast nitrogen base
		+	*deionized water to 900ml
		+	Autoclave for 30 minutes on the liquid cycle. Once cooled to about 55°C add 50ml of the 10x glucose solution and 50ml of the 10x amino acid mix.

	==Notes==		==Notes==

Megan N McClean: /* Contact */

Megan N McClean — Wed, 02 Nov 2011 21:29:00 GMT

Contact

←Older revision		Revision as of 21:29, 2 November 2011
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	<!--Change the information below to your info if you add a new protocol-->		<!--Change the information below to your info if you add a new protocol-->
	*'''[[User:Megan N McClean\|Megan N McClean]] 14:01, 02 November 2011 (EDT)'''		*'''[[User:Megan N McClean\|Megan N McClean]] 14:01, 02 November 2011 (EDT)'''
-
	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].		or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].
		+

	<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->		<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
-	[[Category:Protocol]]	+	[[Category:Protocol]] [[Category:Media]] [[Category:Yeast]]
-	[[Category:Media]]	+
-	[[Category:Yeast]]	+
-		+
-		+
-	~~<!-- Move the relevant categories above this line to tag your protocol with the label~~	+
-	~~[[Category:Protocol]]~~	+
-		+
-	~~-->~~	+

Megan N McClean: /* Contact */

Megan N McClean — Wed, 02 Nov 2011 21:28:30 GMT

Contact

←Older revision		Revision as of 21:28, 2 November 2011
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	==Contact==		==Contact==
	<!--Change the information below to your info if you add a new protocol-->		<!--Change the information below to your info if you add a new protocol-->
-	*'''[[User:Megan N McClean\|Megan N McClean]] 14:01, ~~20 July~~ 2011 (EDT)'''	+	*'''[[User:Megan N McClean\|Megan N McClean]] 14:01, 02 November 2011 (EDT)'''

	or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].		or instead, [[Talk:{{PAGENAME}}\|discuss this protocol]].
Line 53:		Line 53:
	<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->		<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
	[[Category:Protocol]]		[[Category:Protocol]]
		+	[[Category:Media]]
		+	[[Category:Yeast]]
		+

	<!-- Move the relevant categories above this line to tag your protocol with the label		<!-- Move the relevant categories above this line to tag your protocol with the label
	[[Category:Protocol]]		[[Category:Protocol]]

-	~~[[Category:Media]]~~
-
-	~~[[Category:Yeast]]~~
	-->		-->

Megan N McClean: /* Stock Solutions */

Megan N McClean — Wed, 02 Nov 2011 21:27:50 GMT

Stock Solutions

←Older revision		Revision as of 21:27, 2 November 2011
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	==Stock Solutions==		==Stock Solutions==
	''10x Glucose Solution (20% w/v)''		''10x Glucose Solution (20% w/v)''
		+
	For 1 liter of 20% glucose:		For 1 liter of 20% glucose:
	*Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with with constant stirring. Add the water to the beaker first! Heat may help the sugar to dissolve		*Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with with constant stirring. Add the water to the beaker first! Heat may help the sugar to dissolve

Megan N McClean at 21:25, 2 November 2011

Megan N McClean — Wed, 02 Nov 2011 21:25:06 GMT

←Older revision		Revision as of 21:25, 2 November 2011
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	==Stock Solutions==		==Stock Solutions==
-	*10x Glucose Solution (20% w/v)	+	''10x Glucose Solution (20% w/v)''
	For 1 liter of 20% glucose:		For 1 liter of 20% glucose:
-	**Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with with constant stirring. Add the water to the beaker first! Heat may help the sugar to dissolve	+	*Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with with constant stirring. Add the water to the beaker first! Heat may help the sugar to dissolve
-	**Once the sugar has dissolved, bring the entire solution up to 1 liter. Divide into smaller bottles (usually you will use 50mls of 20% glucose to make 1L of media, so we usually aliquot about 100mls into small bottles). Autoclave for 30 minutes on the liquid cycle.	+	*Once the sugar has dissolved, bring the entire solution up to 1 liter. Divide into smaller bottles (usually you will use 50mls of 20% glucose to make 1L of media, so we usually aliquot about 100mls into small bottles). Autoclave for 30 minutes on the liquid cycle.

-	*10x Amino acid mix	+	''10x Amino acid mix''
-	** See [[McClean:KS_Amino_Acid_Supplement \| KS Amino Acid Supplement]]	+	* See [[McClean:KS_Amino_Acid_Supplement \| KS Amino Acid Supplement]]

	==Protocol==		==Protocol==
	For 1 liter of media mix together:		For 1 liter of media mix together:
-	**6.7g yeast nitrogen base	+	*6.7g yeast nitrogen base
-	**20g bacto-agar	+	*20g bacto-agar
-	**deionized water to 900ml	+	*deionized water to 900ml
	Autoclave for 30 minutes on the liquid cycle. Once cooled (but see Notes) to about 55°C add 50ml of the 10x glucose solution and 50ml of the 10x amino acid mix. Pour plates.		Autoclave for 30 minutes on the liquid cycle. Once cooled (but see Notes) to about 55°C add 50ml of the 10x glucose solution and 50ml of the 10x amino acid mix. Pour plates.

Megan N McClean: /* Stock Solutions */

Megan N McClean — Wed, 02 Nov 2011 21:23:43 GMT

Stock Solutions

←Older revision		Revision as of 21:23, 2 November 2011
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	*10x Glucose Solution (20% w/v)		*10x Glucose Solution (20% w/v)
	For 1 liter of 20% glucose:		For 1 liter of 20% glucose:
-	**Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with with constant stirring. Add the water to the beaker first!	+	**Add 200g Dextrose (Sigma D9434) to 400ml of deionized water with with constant stirring. Add the water to the beaker first! Heat may help the sugar to dissolve
-	**Heat may help the sugar to dissolve	+
	**Once the sugar has dissolved, bring the entire solution up to 1 liter. Divide into smaller bottles (usually you will use 50mls of 20% glucose to make 1L of media, so we usually aliquot about 100mls into small bottles). Autoclave for 30 minutes on the liquid cycle.		**Once the sugar has dissolved, bring the entire solution up to 1 liter. Divide into smaller bottles (usually you will use 50mls of 20% glucose to make 1L of media, so we usually aliquot about 100mls into small bottles). Autoclave for 30 minutes on the liquid cycle.

Megan N McClean: /* Stock Solutions */

Megan N McClean — Wed, 02 Nov 2011 21:21:49 GMT

Stock Solutions

←Older revision		Revision as of 21:21, 2 November 2011
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	*10x Amino acid mix		*10x Amino acid mix
-	** See [[McClean:KS_Amino_Acid_Supplement KS Amino Acid Supplement]]	+	** See [[McClean:KS_Amino_Acid_Supplement \| KS Amino Acid Supplement]]

	==Protocol==		==Protocol==