McClean:YNB1: Difference between revisions
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==Protocol== | ==Protocol== | ||
*Container 1: | *Container 1: | ||
** Begin with approx. 700ml MilliQ H<sub>2</sub> | ** Begin with approx. 700ml MilliQ H<sub>2</sub>O in 2L bottle | ||
** Add with constant stirring: 20g of Bacto Agar | ** Add with constant stirring: 20g of Bacto Agar | ||
** Bring up to 800ml with MilliQ | ** Bring up to 800ml with MilliQ H<sub>2</sub>O | ||
** Autoclave | ** Autoclave | ||
** Allow to cool (50°F) | ** Allow to cool (50°F) | ||
*Container 2: | *Container 2: | ||
** Begin with approx. 80ml MilliQ | ** Begin with approx. 80ml MilliQ H<sub>2</sub>O | ||
** Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids) | ** Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids) | ||
** Bring up to 100ml with MilliQ | ** Bring up to 100ml with MilliQ H<sub>2</sub>O | ||
** Autoclave | ** Autoclave | ||
** Allow to cool (50°F) | ** Allow to cool (50°F) | ||
Revision as of 09:51, 2 July 2015
Overview
Yeast nitrogen base without amino acids with agar for plates. This protocol leaves 100ml (per L) of room for additives (glucose, amino acids, etc). Media is labelled as YNB1 on the media shelf.
Reagents
- Yeast Nitrogen Base (without amino acids)
- Bacto Agar
- MilliQ H2O
Protocol
- Container 1:
- Begin with approx. 700ml MilliQ H2O in 2L bottle
- Add with constant stirring: 20g of Bacto Agar
- Bring up to 800ml with MilliQ H2O
- Autoclave
- Allow to cool (50°F)
- Container 2:
- Begin with approx. 80ml MilliQ H2O
- Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids)
- Bring up to 100ml with MilliQ H2O
- Autoclave
- Allow to cool (50°F)
Combine contents of 2 containers and mix well
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
