McClean:YNB3: Difference between revisions
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==Overview== | |||
Yeast nitrogen base without amino acids with agar for plates. This protocol leaves 100ml (per L) of room for additives (glucose, amino acids, etc). Media is labelled as YNB1 on the media shelf. | |||
==Reagents== | |||
• Yeast Nitrogen Base (without amino acids) | |||
• Glucose (sterile 20% solution) | |||
• Bacto Agar | |||
• MilliQ H<sub>2</sub>O | |||
==Protocol== | |||
*Container 1: | *Container 1: | ||
** Begin with approx. 700ml MilliQ H2O in 2L bottle | ** Begin with approx. 700ml MilliQ H2O in 2L bottle | ||
| Line 12: | Line 14: | ||
** Bring up to 800ml with MilliQ H2O | ** Bring up to 800ml with MilliQ H2O | ||
** Autoclave | ** Autoclave | ||
** Allow to cool ( | ** Allow to cool (50°F) | ||
*Container 2: | *Container 2: | ||
** Prepare 100ml solution of 20% Glucose | ** Prepare 100ml solution of 20% Glucose | ||
** Autoclave | ** Autoclave | ||
** Allow to cool ( | ** Allow to cool (50°F) | ||
*Container 3: | *Container 3: | ||
** Begin with approx. 80ml MilliQ H2O | ** Begin with approx. 80ml MilliQ H2O | ||
| Line 22: | Line 24: | ||
** Bring up to 100ml with MilliQ H2O | ** Bring up to 100ml with MilliQ H2O | ||
** Autoclave | ** Autoclave | ||
** Allow to cool ( | ** Allow to cool (50°F) | ||
Combine contents of all 3 containers and mix well | Combine contents of all 3 containers and mix well | ||
==Notes== | |||
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input! | |||
#List troubleshooting tips here. | |||
#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites. | |||
#Anecdotal observations that might be of use to others can also be posted here. | |||
Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip. | |||
==References== | |||
Media Facility at Princeton University at the Lewis-Sigler Institute for Integrative Genomics | |||
==Contact== | |||
*Who has experience with this protocol? | |||
or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. | |||
<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. --> | |||
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[[Category:Protocol]] | |||
[[Category:Media]] | |||
[[Category:Yeast]] | |||
--> | |||
Revision as of 10:55, 30 June 2015
Overview
Yeast nitrogen base without amino acids with agar for plates. This protocol leaves 100ml (per L) of room for additives (glucose, amino acids, etc). Media is labelled as YNB1 on the media shelf.
Reagents
• Yeast Nitrogen Base (without amino acids) • Glucose (sterile 20% solution) • Bacto Agar • MilliQ H2O
Protocol
- Container 1:
- Begin with approx. 700ml MilliQ H2O in 2L bottle
- Add with constant stirring: 20g of Bacto Agar
- Bring up to 800ml with MilliQ H2O
- Autoclave
- Allow to cool (50°F)
- Container 2:
- Prepare 100ml solution of 20% Glucose
- Autoclave
- Allow to cool (50°F)
- Container 3:
- Begin with approx. 80ml MilliQ H2O
- Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids)
- Bring up to 100ml with MilliQ H2O
- Autoclave
- Allow to cool (50°F)
Combine contents of all 3 containers and mix well
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Media Facility at Princeton University at the Lewis-Sigler Institute for Integrative Genomics
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
