McClean:Yeast Transformation

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# 1M LiAC
# 1M LiAC
# 100mM LiAC
# 100mM LiAC
# 50% w/v PEG (mw )
# 50% w/v PEG (mw 3350) (Sigma P3640)
# Sterile H20
# Sterile H20
# Appropriate selective plates (SC-URA, YPD+G418, etc)
# Appropriate selective plates (SC-URA, YPD+G418, etc)

Revision as of 13:14, 20 July 2011



Our lab's version of the Geitz lithium-acetate transformation method.


  1. 1M LiAC
  2. 100mM LiAC
  3. 50% w/v PEG (mw 3350) (Sigma P3640)
  4. Sterile H20
  5. Appropriate selective plates (SC-URA, YPD+G418, etc)

Day 1

  1. Inoculate the strain to transform from a single colony into 5mls of YPD in a test tube. Put on the roller drum at 30°C overnight.

Day 2


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
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  3. Anecdotal observations that might be of use to others can also be posted here.

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Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.


  • Who has experience with this protocol?

or instead, discuss this protocol.

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