Our lab's version of the Geitz lithium-acetate transformation method.
- 1M LiAC
- 100mM LiAC
- 50% w/v PEG (mw )
- Sterile H20
- Appropriate selective plates (SC-URA, YPD+G418, etc)
- Inoculate the strain to transform from a single colony into 5mls of YPD in a test tube. Put on the roller drum at 30°C overnight.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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Adapted from: Kurt Thorn, 3/9/04
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or instead, discuss this protocol.