McClean:Yeast Transformation

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Our lab's version of the Geitz lithium-acetate transformation method.


  1. 1M LiAC
  2. 100mM LiAC
  3. 50% w/v PEG (mw )
  4. Sterile H20
  5. Appropriate selective plates (SC-URA, YPD+G418, etc)

Day 1

  1. Inoculate the strain to transform from a single colony into 5mls of YPD in a test tube. Put on the roller drum at 30°C overnight.

Day 2


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

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Adapted from: Kurt Thorn, 3/9/04


  • Who has experience with this protocol?

or instead, discuss this protocol.

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