McClean:Yeast Transformation

From OpenWetWare

Revision as of 13:14, 20 July 2011 by Megan N McClean (Talk | contribs)
Jump to: navigation, search


Contents

Overview

Our lab's version of the Geitz lithium-acetate transformation method.

Materials

  1. 1M LiAC
  2. 100mM LiAC
  3. 50% w/v PEG (mw 3350) (Sigma P3640)
  4. Sterile H20
  5. Appropriate selective plates (SC-URA, YPD+G418, etc)

Day 1

  1. Inoculate the strain to transform from a single colony into 5mls of YPD in a test tube. Put on the roller drum at 30°C overnight.

Day 2

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


Personal tools