McClean: ConA

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Final concentrations: 5mM MnCl2 5mM CaCl2 2mg/ml concanavalin A
Final concentrations: 5mM MnCl2 5mM CaCl2 2mg/ml concanavalin A
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==Notes==
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
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#List troubleshooting tips here. 
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
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#Anecdotal observations that might be of use to others can also be posted here. 
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
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==Contact==
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*'''[[User:Megan N McClean|Megan N McClean]] 14:01, 20 July 2011 (EDT)'''
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or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].
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[[Category:Media]]
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[[Category:Yeast]]
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Revision as of 17:12, 23 September 2011

Contents

Overview

This is the concanavalin A solution we use for "sticking" yeast cells to the coverslip during microfluidics experiments.

Materials

  • 1M CaCl2 solution in sterile H20
  • 1M MnCl2 solution in sterile H20
  • Concanavalin A (MP Biomedicals, cat no: 150710)

Procedure

Mix 2.5mls of the CaCl2 and the MnCl2 solution in 45mls of water (50ml total volume). Check the pH of this solution. It should be between 6-7. Do not pH this solution with NaOH as the manganese will precipitate out. In this mixture, dissolve a 20mg/ml solution of conA. Dilute from this mixture down to 2mg/ml in miliQ water. Store the 2mg/ml aliquots at -20°C, thaw just before microscopy. Use the 2mg/ml solution to prime flow cells.

Final concentrations: 5mM MnCl2 5mM CaCl2 2mg/ml concanavalin A

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

Contact

or instead, discuss this protocol.


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