McClean: Frogging a Serial Dilution

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Frogging a serial dilution onto solid media is an effective way to distinguish and compare viability under certain growth conditions, etc. The serial dilution helps to eliminate the possibility that a relatively high cell concentration could be misinterpreted as healthy growth, for example.


  • 96-well plate (flat bottom wells)
  • "Frogger"
  • Sterile petri dish about half-full of 95% ethanol
  • Sterile petri dish

Stock Solutions

Stock Solution 1

  • This is a very simple solution, so we only need a one line description of how to make it.

Stock Solution 2

This is a more involved solution, so we will describe how to make it in several steps:

  1. Step 1
  2. Step 2
  3. Step 3


  1. Step 1
  2. Step 2
  3. Step 3


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
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  3. Anecdotal observations that might be of use to others can also be posted here.

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Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.


or instead, discuss this protocol.

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