McClean: NikonTI-Eclipse Setup and operation using Micromanager: Difference between revisions
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(New page: This is the protocol for setting up the Nikon-TI microscope and attached systems for use with Micromanager, but can also be used as a general guide to setting up any system with Micromanag...) |
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Before beginning any setup, it is important to determine exactly all of the different components of the system. Our system includes the following: | Before beginning any setup, it is important to determine exactly all of the different components of the system. Our system includes the following: | ||
*Nikon TI Eclipse Microscope with Perfect Focus System [[Nikon-TI Details|More Details]] | *Nikon TI Eclipse Microscope with Perfect Focus System (Includes a lot of components but you only need to worry about the scope itself)[[Nikon-TI Details|More Details]] | ||
*Andor Zyla sCMOS Camera [http://www.andor.com/pdfs/specifications/Andor_Zyla_sCMOS_Specifications.pdf|More Details] | |||
*2 Lambda SC SmartShutters and Controllers (1 for DIC and 1 for Fluorescence )[http://www.sutter.com/products/sell_sheets/lambda-sc_flyer.pdf|More Details] | |||
*Prior Lumen 200 Fluorescence Illumination System (Is not controlled directly by Micromanager, it's just a lamp)[http://www.prior.com/downloadcentre/documents/brochuresanddatasheets/Lumen200_Datasheet.pdf|More Details] |
Revision as of 08:36, 30 September 2013
This is the protocol for setting up the Nikon-TI microscope and attached systems for use with Micromanager, but can also be used as a general guide to setting up any system with Micromanager.
Hardware Setup
Before beginning any setup, it is important to determine exactly all of the different components of the system. Our system includes the following:
- Nikon TI Eclipse Microscope with Perfect Focus System (Includes a lot of components but you only need to worry about the scope itself)More Details
- Andor Zyla sCMOS Camera Details
- 2 Lambda SC SmartShutters and Controllers (1 for DIC and 1 for Fluorescence )Details
- Prior Lumen 200 Fluorescence Illumination System (Is not controlled directly by Micromanager, it's just a lamp)Details