McClean: PBS/0.1%Tween: Difference between revisions
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| Line 8: | Line 8: | ||
==Protocol== | ==Protocol== | ||
To prepare 1L : | To prepare 1L : | ||
* Add 100 ml 10X PBS to | * Add 100 ml 10X PBS to 899 ml dH2O. | ||
*Add 1 ml Tween-20 and mix. | *Add 1 ml Tween-20 and mix. | ||
Latest revision as of 07:44, 13 April 2012
Overview
We generally use this solution for FACS
Materials
- 10x PBS (see Phosphate Buffered Saline )
- Tween-20 (Fisher BP337-500)
Protocol
To prepare 1L :
- Add 100 ml 10X PBS to 899 ml dH2O.
- Add 1 ml Tween-20 and mix.
