McClean: PlasmidPrep QiagenKit: Difference between revisions
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(New page: <!-- COPY EVERYHING BELOW HERE TO START YOUR OWN PROTOCOL! --> ==Overview== Please make sure to include: 1) Protocol summarized from the Qiagen kit protocol 2) A link to the nanodrop ins...) |
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==Overview== | ==Overview== | ||
Please make sure to include: | Please make sure to include: | ||
*Protocol summarized from the Qiagen kit protocol | |||
* A link to the nanodrop instructions for quantifying your plasmid yield [[McClean: Nanodrop2000 | Using the Nanodrop2000]] | |||
* Any information that is McClean Lab specific (where to get water, where the nanodrop is, etc) | |||
Revision as of 11:57, 27 April 2015
Overview
Please make sure to include:
- Protocol summarized from the Qiagen kit protocol
- A link to the nanodrop instructions for quantifying your plasmid yield Using the Nanodrop2000
- Any information that is McClean Lab specific (where to get water, where the nanodrop is, etc)
Materials
- Item 1
- Item 2
- Stock Solution 1
- Stock Solution 2
Stock Solutions
Stock Solution 1
- This is a very simple solution, so we only need a one line description of how to make it.
Stock Solution 2
This is a more involved solution, so we will describe how to make it in several steps:
- Step 1
- Step 2
- Step 3
Protocol
- Step 1
- Step 2
- Step 3
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. Methods in Enzymology 350: 87-96.
Contact
- Megan N McClean 14:01, 20 July 2011 (EDT)
or instead, discuss this protocol.