McClean: YPD2: Difference between revisions
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(New page: YPD 2 YPD (1x) (1L) Agar • Begin with approx. 700 mls of DI H2O • Weigh out and add ingredients with constant stirring: o Bacto Yeast Extract 10g o Bacto Peptone ...) |
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YPD (1x) (1L) Agar | YPD (1x) (1L) Agar | ||
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• Weigh out and add ingredients with constant stirring: | • Weigh out and add ingredients with constant stirring: | ||
** Bacto Yeast Extract 10g | |||
** Bacto Peptone 20g | |||
• Weigh out and add with stirring: | • Weigh out and add with stirring: | ||
** Bacto Agar 20g | |||
• Bring up to 900 mLs with DI H2O | • Bring up to 900 mLs with DI H2O | ||
Latest revision as of 15:13, 2 June 2015
YPD (1x) (1L) Agar
• Begin with approx. 700 mls of DI H2O • Weigh out and add ingredients with constant stirring:
- Bacto Yeast Extract 10g
- Bacto Peptone 20g
• Weigh out and add with stirring:
- Bacto Agar 20g
• Bring up to 900 mLs with DI H2O • Separate YP into two 1L bottles of 450 mLs each • Weigh out 20g of Glucose/Dextrose and add to 100 mLs of DI H2O • Autoclave separately • Allow to cool in 50û water bath • Combine each 450 mL YP with 50 mLs glucose solution
Verified 6/1/2005 by MaryAnn Hartman; Methods in Yeast Genomics, Cold Spring Harbor, 2000 ed.
