McClean: Yeast Nomenclature
S. cerevisiae researchers have a systematic approach for describing genotypes of yeast strains. We need to follow this system when writing papers, protocols, and when entering yeast strains into the lab database.
Yeast nomenclature is covered well in the articles listed in the references, so I won't recapitulate all of the details here. You should refer to these references when you have questions. I will just list some of the common conventions that you might need when entering strains into the lab database.
Alleles created by recombinant DNA technology
- Deletions sould be written as the name of the gene that is altered following by -Δ; for example arg2-Δ1, where the number 1 denotes a specific complete or partial deletion of arg2.
- Replacements should be written as the name of the gene that is replaced followed by Δ::; For example, we frequently delete the entire open reading frame of a gene with a drug resistance cassette (such as NatMX for ClonNat resistance). If you deleted the GAL1 open reading frame with NatMX you would write gal1Δ::NatMX. If you deleted the GAl1 open reading frame with a fluorescent reporter (for instance, with GFP-KanMX if you wanted a quick reporter of PGAL1 promoter activity) you would write the genotype as ga1Δ::GFP-KanMX
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
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Burke, D. Dawson, D. & Sterns, T. 2000 Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual Cold Spring Harbor Laboratory Press
Sherman, F. 2002 Getting started with yeast Methods Enzymol, 350, 3-41
- Megan N McClean 14:01, 4 June 2012 (EDT)
or instead, discuss this protocol.