McKinney:7H10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: ===Summary=== 7H10 agar is selective medium used for the culture of mycobacteria, in particular ''M. tuberculosis.'' ''M. smegmatis'' is usually grown on LB agar. ===Ingredients=== * 1...) |
mNo edit summary |
||
| Line 7: | Line 7: | ||
* 19g Middlebrook 7H10 powder | * 19g Middlebrook 7H10 powder | ||
* deionized water | * deionized water | ||
* 100mL OADC (Dubos/Middlebrook Oleic Acid Albumin Dextrose Complex) | * 100mL [[McKinney:OADC|OADC]] (Dubos/Middlebrook Oleic Acid Albumin Dextrose Complex) | ||
* 10mL sterile 50% glycerol | * 10mL sterile 50% glycerol | ||
Latest revision as of 14:26, 17 April 2007
Summary
7H10 agar is selective medium used for the culture of mycobacteria, in particular M. tuberculosis. M. smegmatis is usually grown on LB agar.
Ingredients
- 19g Middlebrook 7H10 powder
- deionized water
- 100mL OADC (Dubos/Middlebrook Oleic Acid Albumin Dextrose Complex)
- 10mL sterile 50% glycerol
Protocol
- Mix 19g 7H10 powder in 900mL deionized water in a 2L with a magnetic stirbar until fully dissolved. Top up to 1L with deionized water in a graduated cylinder, then return to the flask and stir until well mixed. Cover with aluminum foil and autoclave.
- Place flasks in a 55°C water bath to cool or cool while stirring with magnetic bar. Prewarm OADC in 37°C water bath.
- In a containment hood, add 100mL OADC and 10mL 50% glycerol to the flask. Replace foil lids, remove from hood, and stir until well mixed. Pipette 24mL per plate into petri dishes. Keep flasks in 55°C water bath until ready to pour.
- Leave plates stacked i nthe hood overnight, then store at 4°C.
